文献类型： 外文期刊

作者： Zheng, Juyun ¹ ; Zhang, Zeliang ¹ ; Liang, Yajun ¹ ; Gong, Zhaolong ¹ ; Zhang, Nala ² ; Ditta, Allah ³ ; Sang, Zhiwei ² ; Wang, Junduo ¹ ; Li, Xueyuan ¹ ;

作者机构： 1.Xinjiang Acad Agr Sci XAAS, Cash Crops Res Inst, Urumqi 830001, Peoples R China

2.Xinjiang Agr Univ, Coll Agr, Engn Res Ctr Cotton, Minist Educ, 311 Nongda East Rd, Urumqi 830052, Peoples R China

3.Nucl Inst Agr & Biol NIAB, Plant Breeding & Genet Div, Cotton Grp, Faisalabad 38000, Pakistan

4.Pakistan Inst Engn & Appl Sci PIEAS, Nucl Inst Agr & Biol, Coll NIAB C, Islamabad 45650, Pakistan

关键词： upland cotton; drought stress; CeRNA; drought resistance gene; hydroponic

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 7 期

页码：

收录情况： SCI

摘要： China, particularly the cotton-growing province of Xinjiang, is experiencing acute agricultural water shortages, stifling the expansion of the cotton sector. Discovering drought resistance genes in cotton and generating high-quality, drought-resistant cotton varieties through molecular breeding procedures are therefore critical to the cotton industry's success. The drought-resistant cotton variety Xinluzhong No. 82 and the drought-sensitive cotton variety Kexin No. 1 were utilised in this study to uncover a batch of drought-resistant candidate genes using whole transcriptome sequencing. The following are the key research findings: A competing endogenous RNA network (ceRNA) was built using complete transcriptional sequencing to screen the core genes in the core pathway, and two drought-related candidate genes were discovered. It was found that gamma-aminobutyric acid aminotransferase (GhGABA-T, Gohir.A11G156000) was upregulated at 0 h vs. 12 h and downregulated at 12 h vs. 24 h. L-Aspartate oxidase (GhAO, Gohir.A07G220600) was downregulated at 0 h vs. 12 h and upregulated at 12 h vs. 24 h. GABA-T is analogous to a pyridoxal phosphate-dependent transferase superfamily protein (POP2) in Arabidopsis thaliana and influences plant drought resistance by controlling gamma-aminobutyric acid (GABA) concentration. The analogue of GhAO in A. thaliana is involved in the early steps of nicotinamide adenine dinucleotide (NAD) production as well as in plant antioxidant responses. This study revealed that gene expression regulatory networks can be used for rapid screening of reliable drought resistance genes and then utilised to validate gene function.