文献类型： 外文期刊

作者： Yan, Huabing ¹ ; Yang, Litao ² ; Li, Yangrui ¹ ;

作者机构： 1.Guangxi Acad Agr Sci GXAAS, Biotechnol Res Inst, Nanning 530007, Guangxi, Peoples R China

2.Guangxi Univ, Coll Agr, Nanning 530007, Guangxi, Peoples R China

关键词： sucrose: 57-50-1;methyl jasmonate: 1211-29-6;activated charcoal;6-benzyladenine: 1214-39-7;paclobutrazol: 76738-62-0;Murashige and Skoog medium: MS medium;naphthalene acetic acid: NAA;26445-01-2;shoot proliferation;shoot dry weight;shoot fresh weight;shoot tuberization

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

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收录情况： SCI

摘要： Dioscorea fordii Prain et Burk., a member of the family Dioscoreaceae, is an important tuberous food in China owing to its edible and medicinal functions. However, the lack of healthy planting material has restricted its production. The present study was an attempt to develop a protocol for in vitro propagation of D. fordii. In an initial two by two (2(2)) factorial experiment, the effects of culture system and activated charcoal have been observed for axillary shoot proliferation and tuberization of in vitro plantlets. Shoot length, frequency of proliferation, fresh weight and dry weight of shoots, frequency of tuberization and mean number of tubers per plantlet (NTPs) were significantly (P a parts per thousand currency sign 0.05) greater in liquid medium compared to semi-solid media. Secondly, an orthogonal experimental design [L-9 (3(4))] was used to investigate the effects of the ratio of naphthalene acetic acid (NAA) to 6-benzyladenine (BA) (NAA/BA), paclobutrazol, methyl jasmonate and sucrose concentration on tuberization. Sucrose concentration had the record effect on frequency of tuberization, NTPs and frequency of proliferation. The preferred medium for axillary shoot proliferation and tuberization of D. fordii found to be MS basal medium (Murashige and Skoog in Physiol Plant 15:473-479, 1962) supplemented with 1.0 mg l(-1) BA, 0.1 mg l(-1) NAA, 30 g l(-1) sucrose and 1.5 g l(-1) AC in liquid culture.