文献类型： 外文期刊

作者： Jiao, Dan ¹ ; Ji, Kaixi ¹ ; Liu, Hu ³ ; Wang, Wenqiang ³ ; Wu, Xiukun ⁴ ; Zhou, Jianwei ³ ; Zhang, Yunsheng ⁶ ; Zhou, Hui ¹ ;

作者机构： 1.Chinese Acad Sci, Northwest Inst Ecol Environm & Resources, Lanzhou 730070, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.Lanzhou Univ, Sch Life Sci, Lanzhou 730070, Peoples R China

4.Chinese Acad Sci, Key Lab Desert & Desertificat, Northwest Inst Ecoenvironm & Resources, Lanzhou 730000, Peoples R China

5.Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Peoples R China

6.Xinjiang Acad Anim Sci, Inst Anim Husb, Xinjiang 830000, Peoples R China

7.Lincoln Univ, Dept Agr Sci, Gene Marker Lab, Lincoln 7647, New Zealand

8.Ben Gurion Univ Negev, Wyler Dept Dryland Agr, Desert Anim Adaptat & Husb, Blaustein Inst Desert Res, IL-18410500 Beer Sheva, Israel

关键词： sheep; cold exposure; transcriptome; adipose tissue

期刊名称：GENES （ 影响因子：3.759； 五年影响因子：3.822 ）

ISSN：

年卷期： 2021 年 12 卷 3 期

页码：

收录情况： SCI

摘要： Thermogenesis plays an important role in the survival of sheep exposed to low temperatures; however, little is known about the genetic mechanisms underlying cold adaptation in sheep. We examined 6 Altay (A) and 6 Hu (H) six-month-old ewe lambs. Altay sheep are raised in northern China and are adapted to dry, cold climates, while Hu sheep are raised in southern China and are adapted to warm, humid climates. Each breed was divided into two groups: chronic cold sheep, exposed to -5 degrees C for 25 days (3 A(C); 3 H(C)), and thermo-neutral sheep, maintained at 20 degrees C (3 A(w); 3 H-w). The transcriptome profiles of hypothalamus, tail-fat and perirenal fat tissues from these four groups were determined using paired-end sequencing for RNA expression analysis. There are differences in cold tolerance between Hu and Altay sheep. Under cold exposure of the lambs: (1) UCP1-dependent thermogenesis and calcium- and cAMP-signaling pathways were activated; and (2) different fat tissues were activated in Hu and Altay lambs. Several candidate genes involved in thermogenesis including UCP1, ADRB3, ADORA2A, ATP2A1, RYR1 and IP6K1 were identified. Molecular mechanisms of thermogenesis in the sheep are discussed and new avenues for research are suggested.