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Study on the Technology of Laparoscopic Ovum Pick-Up and In Vitro Embryo Production in Chongming Goats

文献类型： 外文期刊

作者： Wu, Xiangli 1 ; Li, Dongxu 3 ; Chen, Ying 1 ; Wu, Yangsheng 1 ; Abudureyimu, Gulimire 1 ; Zhang, Wei 1 ; Deng, Kelu 4 ; Huang, Zhen 5 ; Lin, Jiapeng 1 ; Wang, Liqin 1 ;

作者机构： 1.Xinjiang Acad Anim Sci, Inst Biotechnol, Urumqi 830000, Peoples R China

2.Xinjiang Agr Univ, Coll Anim Sci & Technol, Urumqi 830000, Peoples R China

3.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Peoples R China

4.Liuji Yuanzhi Biotechnol Co Ltd, Shanghai 202150, Peoples R China

5.Wanhe Agr Technol Dev Co Ltd, Shanghai 202150, Peoples R China

关键词： laparoscopic ovum pick-up; cumulus-oocyte complexes; in vitro embryo production; ovarian follicular development

期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）

ISSN：

年卷期： 2024 年 13 卷 9 期

页码：

收录情况： SCI

摘要： Simple Summary: This study investigates the use of Chongming goats for optimizing laparoscopic ovum pick-up (LOPU) and in vitro embryo production (IVEP). Key metrics such as the recovery rate of cumulus-oocyte complexes (COCs), the number of ovarian follicles, and the quality of collected COCs are assessed to refine estrus synchronization and superovulation techniques. Additionally, the study examines the effectiveness of LOPU using indicators like cleavage and blastocyst rates to enhance IVEP efficiency, aiming to develop a robust LOPU technology system for small ruminants. The application of laparoscopic ovum pick-up (LOPU) and in vitro production of embryos (IVEP) technologies has opened up a new path for purebred breeding and breed improvement in goats. However, due to the complexity of the procedures and multiple influencing factors, these technologies have not been widely adopted in goat production. This study explores factors affecting the efficiency of LOPU in goats by comparing the use of controlled internal drug release (CIDR) for estrus synchronization, conventional FSH versus long-acting recombinant ovine FSH (R-FSH) for superovulation, and the timing of LOPU at 48 h, 60 h, and 72 h of follicular development. The metrics evaluated included the recovery rate of cumulus-oocyte complexes (COCs), the average number of ovarian follicles, the average number of COCs, and the average number of available COCs. The results demonstrated that the efficiency of LOPU was significantly higher with two doses of R-FSH compared to the conventional FSH superovulation protocol and the control group (p < 0.05), with two doses of R-FSH providing a higher LOPU efficiency than one dose (p < 0.05). Using CIDR for estrus synchronization showed no significant difference in LOPU efficiency compared to the non-CIDR group. Similarly, the efficiency of LOPU showed no significant difference between the hormone treatments at 48 h, 60 h, and 72 h. By exploring and optimizing the factors influencing LOPU, we ultimately established a LOPU technology system for goats that meets the production needs of small ruminants.

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