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DNA methylomes of bovine gametes and in vivo produced preimplantation embryos

文献类型： 外文期刊

作者： Jiang, Zongliang 1 ; Lin, Jianan 2 ; Dong, Hong 6 ; Zheng, Xinbao 6 ; Marjani, Sadie L. 7 ; Duan, Jingyue 8 ; Ouyang, 1 ;

作者机构： 1.Louisiana State Univ, Ctr Agr, Sch Anim Sci, Baton Rouge, LA 70803 USA

2.Jackson Lab Genom Med, Farmington, CT 06032 USA

3.Univ Connecticut, Dept Biomed Engn, Storrs, CT USA

4.Univ Connecticut, Ctr Hlth, Dept Genet & Genome Sci, Farmington, CT USA

5.Univ Connecticut, Ctr Hlth, Inst Syst Genom, Farmington, CT USA

6.Xinjiang Acad Anim Sci, Urumqi, Xinjiang, Peoples R China

7.Cent Connecticut State Univ, Dept Biol, New Britain, CT 06050 USA

8.Univ Connecticut, Dept Anim Sci, Storrs, CT USA

关键词： DNA methylation; gametes; preimplantation embryos; RRBS; bovine

期刊名称：BIOLOGY OF REPRODUCTION （ 影响因子：4.285； 五年影响因子：4.307 ）

ISSN： 0006-3363

年卷期： 2018 年 99 卷 5 期

页码：

收录情况： SCI

摘要： DNA methylation is an important epigenetic modification that undergoes dynamic changes in mammalian embryogenesis, during which both parental genomes are reprogrammed. Despite the many immunostaining studies that have assessed global methylation, the gene-specific DNA methylation patterns in bovine preimplantation embryos are unknown. Using reduced representation bisulfite sequencing, we determined genome-scale DNA methylation of bovine sperm and individual in vivo developed oocytes and preimplantation embryos. We show that (1) the major wave of genome-wide demethylation was completed by the 8-cell stage; (2) promoter methylation was significantly and inversely correlated with gene expression at the 8-cell and blastocyst stages; (3) sperm and oocytes have numerous differentially methylated regions (DMRs)-DMRs specific for sperm were strongly enriched in long terminal repeats and rapidly lost methylation in embryos; while the oocyte-specific DMRs were more frequently localized in exons and CpG islands (CGIs) and demethylated gradually across cleavage stages; (4) DMRs were also found between in vivo and in vitro matured oocytes; and (5) differential methylation between bovine gametes was confirmed in some but not all known imprinted genes. Our data provide insights into the complex epigenetic reprogramming of bovine early embryos, which serve as an important model for human preimplantation development. Summary Sentence Genome-wide gene-specific DNA methylation in bovine sperm, oocytes and preimplantation embryos.

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