文献类型： 外文期刊

作者： Li, Zhuanjian ¹ ; Guo, Ruoting ² ; Gu, Zhenzhen ⁴ ; Wang, Xiangnan ¹ ; Wang, Yongcai ¹ ; Xu, Huifen ¹ ; Wang, Chunxiu; ¹ ;

作者机构： 1.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China

2.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Xinjiang, Peoples R China

3.Ctr Anim Dis Control & Prevent, Changji 831100, Xinjiang, Peoples R China

4.Xinjiang Acad Anim Sci, Minist Agr, Key Lab Genet Breeding & Reprod Grass Feeding Liv, Urumqi 830026, Xinjiang, Peoples R China

关键词： Sheep; GnRH; Otx-2; Promoter activity; Transcriptional regulation

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2019 年 699 卷

页码：

收录情况： SCI

摘要： Gonadotropin-releasing hormone (GnRH) plays an important role in regulating the activities of other components downstream of the hypothalamic-pituitary-gonadal (HPG) axis and maintaining the normal reproductive cycle of animals. However, the molecular mechanisms by which GnRH synthesis and secretion are regulated in sheep remains unclear. In this study, a series of eight recombinant vectors with deletion fragments were constructed and cotransfected with pGL3-Basic and pRL-SV40 into sheep hypothalamic neuronal cells. After treatment with 1 nM kisspeptin, the core promoter of the sheep GnRH gene was identified to be in the region of -1912 bp to -1461 bp by dual-luciferase reporter assay. Bioinformatics analysis showed that there was a binding site for the transcription factor Otx-2 in the core promoter region (- 1786 to -1770 bp) that was highly conserved among different species. The expression patterns of Kiss-1, Otx-2 and GnRH in the sheep hypothalamus were the same, and the expression of Kiss-1, Otx-2 and GnRH was significantly higher in the breeding season than in nonbreeding season (P < 0.01). In addition, when hypothalamic neurons were cultured in vitro with kisspeptin, kisspeptin induced the expression of GnRH and Otx-2. In conclusion, these results provide evidence that the core promoter region (-1786 to -1770 bp) of the GnRH gene is involved in the regulation of hypothalamic activity by kisspeptin and that binding of the transcription factor Otx-2 mediates this activation.