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Vitamin determination in pet food: A fast and sensitive online solid-phase extraction and heart-cutting 2D liquid chromatography method

文献类型： 外文期刊

作者： Li, Yang 1 ; Wang, Jitong 2 ; Gong, Ping 3 ; Feng, Yuchao 1 ; Yao, Ting 2 ; Gao, Xing 4 ; Guo, Wei 4 ; Chen, Xue 1 ; Cui, Jie 1 ; Fan, Xia 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China

2.Beijing Vet Drug & Feed Monitoring Ctr, Beijing 102200, Peoples R China

3.Xinjiang Acad Anim Sci, Inst Anim Husb Qual Stand, Urumqi Xinjiang 830057, Peoples R China

4.Acchrom Technol Co Ltd, Beijing 101407, Peoples R China

关键词： Vitamin A; Vitamin D; Vitamin E isomers; Pet food; Online solid-phase extraction; Two-dimensional liquid chromatography

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）

ISSN： 0026-265X

年卷期： 2025 年 213 卷

页码：

收录情况： SCI

摘要： An automatic method that combines online solid-phase extraction (online SPE) and heart-cutting two-dimensional liquid chromatography (2D-LC) was developed to determine seven fat-soluble vitamins simultaneously and was validated in pet food. The saponified sample solution was injected directly into our combined online SPE and heart-cut 2D-LC system for enrichment, purification, separation, and quantification. Vitamin A and E isomers were analyzed using pentafluorophenyl (PFP) as the first column. Vitamins D-2 and D-3 were analyzed using C-18 as the second column. The results showed good linearity (R-2 > 0.9964) for the analytes and good reproducibility (RSD < 3 %, n = 3). The limits of quantification for vitamins A, D-2, and D-3, and alpha -, beta-, gamma-, and delta-E (calculated using S/N = 10:1) were 0.24, 0.09, 0.09, 2.4, 2.4, 2.4 and 2.4 mu g/g, respectively. The spiked recoveries were from 80.6 to 104.0 %. The proposed method considerably simplifies the analytical workflow of standard methods for the analysis of fat-soluble vitamins and improves efficiency.

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