文献类型： 外文期刊

作者： Abdurahman, Anwar ¹ ; Aierken, Wusimanjiang ⁴ ; Zhang, Fei ⁵ ; Obulkasim, Rahmantay ⁶ ; Aniwashi, Jueken ⁷ ; Sulayman, Ablat ⁸ ;

作者机构： 1.Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Bioresources & Ecol, Shenzhen, Peoples R China

2.Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen, Peoples R China

3.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing, Peoples R China

4.Xinjiang Med Univ, Affiliate Hosp 5, Urumqi, Peoples R China

5.Anim Dis Control & Prevent Ctr Xinjiang Uygur Auto, Urumqi, Peoples R China

6.Ili Kazak Autonomous Prefecture Inst Anim Sci, Ili, Peoples R China

7.Xinjiang Agr Univ, Coll Anim Sci & Technol, Urumqi, Peoples R China

8.Xinjiang Acad Anim Sci, Inst Anim Husb, Urumqi, Peoples R China

关键词： BMPR1B; granulosa cell; miR-1306; apoptosis; sheep

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.772； 五年影响因子：4.933 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Bone morphogenetic protein receptor type-1B (BMPR1B) is one of the major gene for sheep prolificacy. However, few studies investigated its regulatory region. In this study, we reported that miR-1306 is a direct inhibitor of BMPR1B gene in the ovine granulosa cells (ovine GCs). We detected a miRNA response element of miR-1306 in the 3' untranslated region of the ovine BMPR1B gene. Luciferase assay showed that the ovine BMPR1B gene is a direct target of miR-1306. qPCR and western blotting revealed that miR-1306 reduces the expression of BMPR1B mRNA and protein in the ovine granulosa cells. Furthermore, miR-1306 promoted cell apoptosis by suppressing BMPR1B expression in the ovine granulosa cells. Overall, our results suggest that miR-1306 is an epigenetic regulator of BMPR1B, and may serve as a potential target to improve the fecundity of sheep.