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CircERCC6 Positively Regulates the Induced Activation of SHF Stem Cells in Cashmere Goats via the miR-412-3p/BNC2 Axis in an m6A-Dependent Manner

文献类型： 外文期刊

作者： Zhang, Qi 1 ; Fan, Yixing 1 ; Bai, Man 1 ; Zhu, Yubo 1 ; Wang, Zeying 1 ; Shen, Jincheng 1 ; Xu, Ruqing 1 ; Zheng, Wenxin 2 ; Bai, Wenlin 1 ; Bagnicka, Emilia 1 ; Einspanier, Ralf 1 ;

作者机构： 1.Shenyang Agr Univ, Coll Anim Sci & Vet Med, Shenyang 110866, Peoples R China

2.Minist Sci & Technol & Xinjiang Uygur Autonomous R, State Key Lab Herbivorous Livestock Genet Improvem, Urumqi 830011, Peoples R China

3.Xinjiang Acad Anim Sci, Urumqi 830011, Peoples R China

关键词： CircERCC6; N-6-Methyladenosine; SHF stem cells; induced activation; cashmere goats

期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 2 期

页码：

收录情况： SCI

摘要： The cashmere, a kind of nature protein fiber, is one of the main use of cashmere goats. The induced activation of secondary hair follicle (SHF) stem cells by the dermal papilla cell-derived signals is a key biological process for the morphogenesis and growth of cashmere fiber in cashmere goats. Previously, the circRNA-ERCC6 (circERCC6) was identified from cashmere goat SHFs; however, its biological significance is unclear in the SHF physiology process of cashmere goats. In this study, we found that circERCC6 exhibited significantly higher expression at anagen SHF bulge compared with the counterpart of telogen and harbored three m(6)A modified sites (named m(6)A-685, m(6)A-862, and m(6)A-995) through methylation immunoprecipitation using a real-time quantitative polymerase chain reaction (Me-RIP-qPCR) technique. The knockdown experiments of circERCC6 in SHF stem cells showed that circERCC6 positively regulates the induced activation of SHF stem cells in cashmere goats. Through a dual-luciferase reporter assay, we demonstrated that m(6)A-modified circERCC6 (m(6)A-circERCC6) sponged miR-412-3p to upregulate the expression of BNC2 mRNA in SHFstem cells. Through m(6)A-deficient mutant assay in circERCC6 knockdown SHF stem cells, we further showed that m(6)A modification within circERCC6 is required to mediate the miR-412-3p/BNC2 axis to finally promote the proper induced activation of SHF stem cells in cashmere goats.

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