文献类型： 外文期刊

作者： Sharma, Anjney ¹ ; Song, Xiu-Peng ² ; Singh, Rajesh Kumar ¹ ; Vaishnav, Anukool ³ ; Gupta, Saurabh ³ ; Singh, Pratiksha ¹ ; Guo, Dao-Jun ¹ ; Verma, Krishan K. ¹ ; Li, Yang-Rui ¹ ;

作者机构： 1.Guangxi Acad Agr Sci GXAAS, Chinese Acad Agr Sci, Sugarcane Res Ctr, Key Lab Sugarcane Biotechnol & Genet Improvement G, Nanning, Guangxi, Peoples R China

2.Guangxi Acad Agr Sci GXAAS, Sugarcane Res Inst, Guangxi Key Lab Sugarcane Genet Improvement, Nanning, Guangxi, Peoples R China

3.GLA Univ, Dept Biotechnol, Mathura, Uttar Pradesh, India

4.Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobio, Nanning, Guangxi, Peoples R China

关键词： carbendazim; PGPR; CLSM; reactive oxygen species; oxidative stress; antioxidant enzymes

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Agrochemicals are consistently used in agricultural practices to protect plants from pathogens and ensure high crop production. However, their overconsumption and irregular use cause adverse impacts on soil flora and non-target beneficial microorganisms, ultimately causing a hazard to the ecosystem. Taking this into account, the present study was conducted to determine the high dosage of fungicide (carbendazim: CBZM) effects on the rhizobacteria survival, plant growth promoting trait and reactive oxygen species (ROS) scavenging antioxidant enzyme system. Thus, a multifarious plant growth promoting rhizobacteria (PGPR) isolate, ANCB-12, was obtained from the sugarcane rhizosphere through an enrichment technique. The taxonomic position of the isolated rhizobacteria was confirmed through 16S rRNA gene sequencing analysis as Priestia megaterium ANCB-12 (accession no. ). Results showed that increasing concentrations of fungicide showed adverse effects on rhizobacterial cell growth and survival. In addition, cell visualization under a confocal laser scanning microscope (CLSM) revealed more oxidative stress damage in the form of ROS generation and cell membrane permeability. Furthermore, the increasing dose of CBZM gradually decreased the plant growth promoting activities of the rhizobacteria ANCB-12. For example, CBZM at a maximum 3,000 mu g/ml concentration decreases the indole acetic acid (IAA) production by 91.6%, ACC deaminase by 92.3%, and siderophore production by 94.1%, respectively. Similarly, higher dose of fungicide enhanced the ROS toxicity by significantly (p < 0.05) modulating the stress-related antioxidant enzymatic biomarkers in P. megaterium ANCB-12. At a maximum 3,000 mu g/ml CBZM concentration, the activity of superoxide dismutase (SOD) declined by 82.3%, catalase (CAT) by 61.4%, glutathione peroxidase (GPX) by 76.1%, and glutathione reductase (GR) by 84.8%, respectively. The results of this study showed that higher doses of the fungicide carbendazim are toxic to the cells of plant-beneficial rhizobacteria. This suggests that a recommended dose of fungicide should be made to lessen its harmful effects.