Detection of single nucleotide polymorphism in HTR4 and its relationship with growth traits in sheep
文献类型： 外文期刊
作者： Xu, Dan 1 ; Wang, Xiaojuan 1 ; Wang, Weimin 2 ; Zhang, Deyin 2 ; Li, Xiaolong 2 ; Zhang, Yukun 2 ; Zhao, Yuan 2 ; Cheng, Jiangbo 1 ; Zhao, Liming 1 ; Wang, Jianghui 1 ; Lin, Changchun 1 ; Yang, Xiaobin 1 ; Weng, Xiuxiu 2 ; Zhang, Xiaoxue 1 ; Zheng, Wenxin 3 ;
作者机构： 1.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou 730030, Peoples R China
2.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou, Peoples R China
3.Xinjiang Acad Anim Sci, Inst Anim Husb Qual Stand, Urumqi 830000, Xinjiang, Peoples R China
关键词： HTR4; growth traits; SNP; qRT-PCR; sheep
期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：3.7； 五年影响因子：3.3 ）
ISSN： 1049-5398
年卷期： 2023 年
页码：
收录情况： SCI
摘要： In this study, a single nucleotide polymorphism of HTR4 (hydroxytryptamine receptor 4) was detected using DNA sequencing and KASPar (Kompetitive Allele-Specific PCR) technique with the aim of analyzing its effect on growth traits in 1102 sheep. A synonymous mutation g.101220 C> T located on the fifth intron of the ovis HTR4 gene was detected, and association analysis showed that this mutation was significantly associated with growth traits in sheep (p < .05), with TT genotypes having significantly lower body weight, height, length and chest circumference than TC and CC genotypes. It showed that the polymorphism of this locus was significantly associated with growth traits in sheep. In addition, qRT-PCR results showed that HTR4 was expressed in different tissues of sheep. It is highly expressed in the liver, spleen and duodenum. As important metabolic, immune and digestive absorption organs in animals, the above tissues can regulate the excitability of intestinal smooth muscle by participating in the body metabolism and nutrient metabolism of sheep, so that sheep can show better growth characteristics. In conclusion, the polymorphic locus identified in HTR4 gene can be used as candidate molecular marker in sheep breeding.
- 相关文献
作者其他论文 更多>>
-
Rapid detection and spectroscopic feature analysis of mineral content in camel milk using fourier-transform mid-infrared spectroscopy and traditional machine learning algorithms
作者：Li, Yongqing;Fan, Yikai;Gao, Jingyi;Liu, Li;Wang, Haitong;Chu, Chu;Yang, Zhuo;Yang, Guochang;Wen, Peipei;Wang, Dongwei;Zhang, Shujun;Li, Yongqing;Liu, Li;Cao, Lijun;Hu, Bo;Abula, Zunongjiang;Xieermaola, Yeerlan;Zheng, Wenxin
关键词：Camel milk; Minerals; FT-MIRS; Machine learning; Detection method
-
Rapid identification of scoured protein fibers using near-infrared spectroscopy with machine learning: A comparison of handheld and benchtop devices
作者：Gong, Ping;Wei, Peiling;Xu, Yanli;Zhang, Rongyin;Zheng, Wenxin;Fan, Xia;Feng, Yuchao;Fan, Xia
关键词：Cashmere; Wool; Convolutional neural networks; Handheld near-infrared spectrometer
-
Spatial heterogeneity determines the gastrointestinal microbiome signatures and ecological processes that govern bacterial community assembly in sheep
作者：Zhang, Yukun;Cheng, Jiangbo;Li, Fadi;Zhang, Deyin;Yang, Xiaobin;Xu, Dan;Zhao, Yuan;Zhao, Liming;Li, Xiaolong;Tian, Huibin;Weng, Xiuxiu;Wang, Weimin;Zhang, Yukun;Cheng, Jiangbo;Li, Fadi;Zhang, Deyin;Yang, Xiaobin;Xu, Dan;Zhao, Yuan;Zhao, Liming;Li, Xiaolong;Tian, Huibin;Weng, Xiuxiu;Wang, Weimin;Zhang, Yukun;Cheng, Jiangbo;Li, Fadi;Zhang, Deyin;Yang, Xiaobin;Xu, Dan;Zhao, Yuan;Zhao, Liming;Li, Xiaolong;Tian, Huibin;Weng, Xiuxiu;Wang, Weimin;Zhang, Yukun;Cheng, Jiangbo;Li, Fadi;Zhang, Deyin;Yang, Xiaobin;Xu, Dan;Zhao, Yuan;Zhao, Liming;Li, Xiaolong;Tian, Huibin;Weng, Xiuxiu;Wang, Weimin;Lin, Changchun;Zhang, Xiaoxue;Li, Chong
关键词：sheep; gastrointestinal tract; co-occurrence networks; enterotypes; bacterial community assembly
-
Ovine TUSC5 and IGFBP3 Gene Polymorphisms and their Association with Tail Fat Weight in Sheep
作者：Cao, Peiliang;He, Lijuan;Jia, Guoxing;Kong, Dewen;Li, Linting;Zhang, Jian;Li, Hongjian;Zhang, Xiaoxue;Zhang, Deyin;Xu, Dan;Ma, Zongwu;Pu, Mengru;Li, Fadi;Tian, Huibin;Wang, Weimin;Wu, Weiwei
关键词：IGFBP3; sheep; SNPs; TUSC5; tail fat weight
-
Novel strategy for effective selection of characteristic spectra of milk minerals
作者：Liu, Li;Yang, Zhuo;Xu, Gang;Fan, Yikai;Li, Yongqing;Zuo, Bo;Zhang, Shujun;Liu, Li;Yang, Zhuo;Xu, Gang;Fan, Yikai;Li, Yongqing;Zhang, Shujun;Liu, Li;Li, Yongqing;Cao, Lijun;Hu, Bo;Abula, Zunongjiang;Zheng, Wenxin
关键词：Milk; Mineral; Mid-infrared spectroscopy; Predictive model; Random frog
-
Identification of candidate genes for reproductive traits in Xinjiang sheep breeds based on genomic structural variation
作者：Li, Jiaqi;Yiming, Sulaiman;Zheng, Wenxin;Bi, Jingdong;Li, Fengming;Zhu, Mengting;Wu, Weiwei
关键词：sheep; whole-genome resequencing; structural variation; reproductive traits; selection signals
-
Fatty acid composition and origin of frozen lamb determined by handheld NIR spectroscopy with chemometrics
作者：Feng, Yuchao;Fan, Xia;Wei, Peiling;Zheng, Wenxin;Gong, Ping;Fan, Xia;Zhang, Yushu
关键词：Frozen lamb; Fatty acid; Partial least squares regression; Partial least squares discriminant analysis; Handheld near-infrared spectrometer
