文献类型： 外文期刊

作者： Liu, Dan ¹ ; Wang, Yuan-Zhi ² ; Zhang, Huan ¹ ; Liu, Zhi-Qiang ³ ; Wureli, Ha-Zi ¹ ; Wang, Shi-Wei ⁴ ; Tu, Chang-Chun; ¹ ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832002, Xinjiang Uygur, Peoples R China

2.Shihezi Univ, Sch Med, Shihezi 832002, Xinjiang Uygur, Peoples R China

3.Xinjiang Acad Anim Sci, Inst Vet Med, Urumqi 830000, Xinjiang Uygur, Peoples R China

4.Tarim Univ, Coll Anim Sci, Alar 843300, Xinjiang Uygur, Peoples R China

5.Acad Mil Med Sci, Inst Vet Sci, Changchun 130062, Jilin, Peoples R China

关键词： Melophagus ovinus;Rickettsia raoultii;Rickettsia slovaca;China

期刊名称：PARASITES & VECTORS （ 影响因子：3.876； 五年影响因子：3.959 ）

ISSN： 1756-3305

年卷期： 2016 年 9 卷

页码：

收录情况： SCI

摘要： Background: Melophagus ovinus (Diptera: Hippoboscidae), a hematophagous ectoparasite, is mainly found in Europe, Northwestern Africa, and Asia. This wingless fly infests sheep, rabbits, and red foxes, and causes inflammation, wool loss and skin damage. Furthermore, this parasite has been shown to transmit diseases, and plays a role as a vector. Herein, we investigated the presence of various Rickettsia species in M. ovinus. Methods: In this study, a total of 95 sheep keds were collected in Kuqa County and Alaer City southern region of Xinjiang Uygur Autonomous Region, northwestern China. First, collected sheep keds were identified on the species level using morphological keys and molecular methods based on a fragment of the 18S ribosomal DNA gene (18S rDNA). Thereafter, to assess the presence of rickettsial DNA in sheep keds, the DNA of individual samples was screened by PCR based on six Rickettsia-specific gene fragments originating from six genes: the 17-kilodalton antigen gene (17-kDa), 16S rRNA gene (rrs), surface cell antigen 4 gene (sca4), citrate synthase gene (gltA), and outer membrane protein A and B genes (ompA and ompB). The amplified products were confirmed by sequencing and BLAST analysis (https://blast. ncbi. nlm. nih. gov/Blast. cgi? PROGRAM= blastn& PAGE_ TYPE= BlastSearch& LINK_ LOC= blasthome). Results: According to its morphology and results of molecular analysis, the species was identified as Melophagus ovinus, with 100% identity to M. ovinus from St. Kilda, Australia (FN666411). DNA of Rickettsia spp. were found in 12 M. ovinus samples (12.63%, 12/95). Rickettsia raoultii and R. slovaca were confirmed based on phylogenetic analysis, although the genetic markers of these two rickettsial agents amplified in this study showed molecular diversity. Conclusions: This is the first report of R. raoultii and R. slovaca DNA in M. ovinus. Rickettsia slovaca was found for the first time around the Taklimakan Desert located in China. This finding extends the geographical range of spotted fever group rickettsiae.