In vitro culture of Echinococcus multilocularis producing protoscoleces and mouse infection with the cultured vesicles
文献类型： 外文期刊
作者： Wang, Hui 1 ; Li, Jun 1 ; Guo, Baoping 1 ; Zhao, Li 2 ; Zhang, Zhuangzhi 2 ; McManus, Donald P. 3 ; Wen, Hao 1 ; Zhang, W 1 ;
作者机构： 1.Xinjiang Med Univ, Affiliated Hosp 1, Clin Med Res Inst, State Key Lab Incubat Base Xinjiang Major Dis Res, Urumqi 830054, Xinjiang, Peoples R China
2.Xinjiang Acad Anim Sci, Vet Res Inst, Urumqi 830000, Xinjiang, Peoples R China
3.QIMR Berghofer Med Res Inst, Div Infect Dis, Mol Parasitol Lab, Brisbane, Qld, Australia
关键词： Echinococcus multilocularis;Protoscoleces;Vesicles;In vitro culture;Animal model
期刊名称：PARASITES & VECTORS （ 影响因子：3.876； 五年影响因子：3.959 ）
ISSN： 1756-3305
年卷期： 2016 年 9 卷
页码：
收录情况： SCI
摘要： Background: Alveolar echinococcosis (AE) is a lethal zoonosis caused by the fox-tapeworm Echinococcus multilocularis. The disease is difficult to treat and an effective therapeutic drug is urgently needed. Reliable models are essential for drug development. In this study, we developed both in vitro and in vivo models of larval E. multilocularis. Results: The protoscoleces (PSC) of E. multilocularis from jirds were successfully cultured in a modified RPMI1640 based medium containing 25 % (v/v) fetal bovine serum (FBS). After 100 days of culture, PSC developed to larval vesicles (small unilocular cysts) and the fast growing vesicles produced PSC in brood capsules. In addition, mice were intraperitoneally injected with 30 cultured small vesicles and 100 % of the mice had resulting metacestode masses. Conclusions: Larval protoscoleces and vesicles of E. multilocularis grow healthily in vitro in the RPMI1640 based medium containing 25 % FBS. Echinococcus multilocularis in vitro and in vivo models provide a valuable platform for investigating the biology of the parasite and screening effective therapeutic drugs against AE.
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