文献类型： 外文期刊

作者： Du, W. ¹ ; Xia, J. ² ; Zhang, Y. ² ; Liu, M. J. ¹ ; Li, H. B. ² ; Yan, X. M. ² ; Zhang, J. S. ² ; Li, N. ² ; Zhou, Z. Y. ² ; Xi ¹ ;

作者机构： 1.Shihezi Univ, Coll Anim Sci, Shihezi, Peoples R China

2.Xinjiang Acad Anim Sci, Urumqi, Peoples R China

关键词： Myostatin propeptide;Recombinant plasmid;Biological activity

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： Myostatin propeptide can inhibit the biological activity of myostatin protein and promote muscle growth. To express myostatin propeptide in vitro with a higher biological activity, we performed codon optimization on the sheep myostatin propeptide gene sequence, and mutated aspartic acid-76 to alanine based on the codon usage bias of Pichia pastoris and the enhanced biological activity of myostatin propeptide mutant. Modified myostatin propeptide gene was cloned into the pPIC9K plasmid to form the recombinant plasmid pPIC9K-Msp. Recombinant plasmid pPIC9K-Msp was transformed into Pichia pastoris GS115 by electrotransformation. Transformed cells were screened, and methanol was used to induce expression. SDS-PAGE and western blotting were used to verify the successful expression of myostatin propeptide with biological activity in Pichia pastoris, providing the basis for characterization of this protein.