文献类型： 外文期刊
作者： Liu Wu-Jun 2 ; Wang Qiong 2 ; Huang Xi-Xia 2 ; Yu Shi-Gang 2 ; Tian Ke-Chuan 1 ;
作者机构： 1.Xinjiang Acad Anim Sci, Urumqi 830000, Xinjiang, Peoples R China
2.Xinjiang Agr Univ, Coll Anim Sci, Urumqi 830052, Xinjiang, Peoples R China
关键词： KIF-1 gene;PCR-RFLP;polymorphism;SNP;genotype;frequencies
期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）
ISSN： 1680-5593
年卷期： 2011 年 10 卷 5 期
页码：
收录情况： SCI
摘要： Keratin Intermediate-Filament (KIF) proteins form the main structural elements of a wool fibre. In this study, PCR-RFLP and DNA sequencing methods were used to detect polymorphisms of KIF-1 gene in 786 samples of 3 Xinjiang local goat breeds (Xinjiang, Nanjiang Cashmere and Bogeda Cashmere goat). The results showed that KIF-1-P3 locus has polymorphisms. The frequencies of genotype AA were 0.675, 0.577 and 0.766, genotype AB were 0.296, 0.400 and 0.234, genotype BB were 0.030, 0.023 and 0.000 for Xinjiang, Nanjiang Cashmere and Bogeda Cashmere goat breeds, respectively. The genotypic distributions were in disagreement with Hardy-Weinberg equilibrium in Nanjiang Cashmere goat breed. In addition, the mutation was detected at KIF-1-P3 locus by DNA sequencing method, a novel of SNP was revealed in. exon 3 (Genebank M23912: c.3212 C>T) and it belongs to synonymous mutation. However, there is no significant difference in. Xinjiang goat breed between polymorphisms of the KIF-1 gene and cashmere production traits (fineness, thickness, yield and body weight after combing). Moreover, analysis of variance indicated that various genotypes in the 3 Xinjiang goat breeds were highly significant (p<0.01).
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