文献类型： 外文期刊

作者： Pan, Y ¹ ; Cai, H ² ; Li, SX ² ; Tian, X ² ; Li, T ² ; Zhu, YX ² ;

作者机构： 1.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China

2.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China; Xinjiang Acad Anim Sci, Urumqi 830000, Peoples R China

关键词： Mycobacterium tuberculosis;secreted proteins;DNA vaccine;protective efficacy

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 0582-9879

年卷期： 2003 年 35 卷 1 期

页码：

收录情况： SCI

摘要： Three proteins secrected from Mycobacterium tuberculosis, Ag85B, ESAT-6 and MPT63 were seclected as antigens for making combined DNA vaccine by immunizing mice. The immune response induced by the vaccine and its protective efficacy were studied. It was demonstrated that when mice were immunized with the combined DNA vaccine, the titer of antibody for Ag85B in serum increased to more than 10(5), but the titers of ESAT-6 and MPT63 specific antibodies were undetectable. After the final immunization, the level of IFN-gamma specific for Ag85B, ESAT-6 and MPT63 reached (17.0 +/- 7. 0) u/ml, (6.0 +/- 0.8) u/ml and (11.9 +/- 8.0) u/ml, respectively. Mice, that were inoculated with the empty eukaryotic expression vector pJW4303 DNA, produced negligible amounts of antigen-specific INF-gamma. The combined DNA vaccine resulted also in significantly reduced amount of bacteria in the lungs of experimental mice. Microphotographs showed clearly that these lungs were better protected against Mycobacterium tuberculosis challenge than control mice. The combined DNA vaccine reported in this work shed new lights on the prophylactid protection against tuberculosis.