文献类型： 外文期刊

作者： Tuerhong, Reyilanmu ¹ ; Xin, Lianxi ¹ ; Zhang, Ying ¹ ; Tuoheti, Abudusaimaiti ¹ ; Zhang, Yi ¹ ; Maimaiti, Ailixire ³ ; Abula, Saifuding ¹ ; Wusiman, Adelijiang ¹ ; Aimulajiang, Kalibixiati ⁴ ; Tuersong, Waresi ¹ ;

作者机构： 1.Xinjiang Agr Univ, Coll Vet Med, Urumqi 830052, Xinjiang, Peoples R China

2.Xinjiang Agr Univ, Xinjiang Key Lab New Drug Study & Creat Herbivorou, Urumqi 830052, Peoples R China

3.Xinjiang Acad Anim Sci, Xinjiang Vet Res Inst, Urumqi 830099, Peoples R China

4.Xinjiang Med Univ, Affiliated Hosp 1, Clin Med Inst, State Key Lab Pathogenesis Prevent & Treatment Hig, Urumqi 830011, Peoples R China

关键词： Haemonchus contortus; Benzimidazole resistance; Isotype-1 beta-tubulin gene; Single-nucleotide polymorphism

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：1.8； 五年影响因子：1.9 ）

ISSN： 0932-0113

年卷期： 2024 年 123 卷 8 期

页码：

收录情况： SCI

摘要： To understand the benzimidazole (BZ) resistance of Haemonchus contortus in Southern Xinjiang, three single nucleotide polymorphisms (SNPs) designated as F167Y, E198A, and F200Y, in the isotype-1 beta-tubulin gene which are associated with BZ resistance, were investigated for H. contortus populations from sheep in Hejing and Minfeng counties of Southern Xinjiang. In brief, a total of 190 H. contortus adults were collected from 52 out of 70 slaughtered sheep in city abattoirs across two regions in Southern Xinjiang. The species identity of each adult worm was confirmed by PCR amplification of ITS-2 using H. contortus-specific primers targeting the ITS-2. The samples were then investigated for BZ-related SNPs at locus 167, 198, and 200, by PCR-sequencing of the isotype-1 beta-tubulin gene. The results showed that only E198A and F200Y mutations were detected in the investigated H. contortus populations. The E198A mutation (homozygous and heterozygote resistant: found in 40% and 30% of sequenced samples from Minfeng and Hejing counties, respectively) was predominant compared with the F200Y mutation (homozygous and heterozygote resistant: found in 14% and 13.3% of sequenced samples from Minfeng and Hejing counties, respectively). The results indicate a high prevalence of BZ resistance in H. contortus populations from certain areas of Southern Xinjiang. Our findings provide valuable information for the prevention and control of H. contortus in areas with similar conditions.