Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells
文献类型： 外文期刊
作者： Ma, Yingcai 1 ; Guo, Xueping 1 ; He, Qin 1 ; Liu, Lu 1 ; Li, Zelong 1 ; Zhao, Xiaomin 3 ; Gu, Wenxi 4 ; Zhong, Qi 4 ; Li, Na 1 ; Yao, Gang 1 ; Ma, Xuelian 1 ;
作者机构： 1.Xinjiang Agr Univ, Coll Vet Med, Urumqi 830052, Peoples R China
2.Xinjiang Agr Univ, Xinjiang key Lab New Drug Study & Creat Herbivorou, Urumqi 830052, Peoples R China
3.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China
4.Xinjiang Acad Anim Sci, Inst Anim Sci, Urumqi 830011, Peoples R China
关键词： Infectious bovine rhinotracheitis virus (IBRV); MicroRNA (miRNA); miR-10a; miR-182; Mitochondrial damage
期刊名称：BMC GENOMICS （ 影响因子：4.4； 五年影响因子：4.7 ）
ISSN： 1471-2164
年卷期： 2024 年 25 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundStudies have confirmed that Infectious bovine rhinotracheitis virus (IBRV) infection induces mitochondrial damage. MicroRNAs (miRNAs) are a class of noncoding RNA molecules, which are involved in various biological processes and pathological changes associated with mitochondrial damage. It is currently unclear whether miRNAs participate in IBRV-induced mitochondrial damage in Madin-Darby bovine kidney (MDBK) cells.ResultsIn the present study, we used high-throughput sequencing technology, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to screen for mitochondria-related miRNAs and messenger RNAs (mRNAs). In total, 279 differentially expressed miRNAs and 832 differentially expressed mRNAs were identified in 6 hours (IBRV1) versus 24 hours (IBRV2) after IBRV infection in MDBK cells. GO and KEGG enrichment analysis revealed that 42 differentially expressed mRNAs and 348 target genes of differentially expressed miRNAs were correlated with mitochondrial damage, and the miRNA-mitochondria-related target genes regulatory network was constructed to elucidate their potential regulatory relationships. Among the 10 differentially expressed miRNAs, 8 showed expression patterns consistent with the high-throughput sequencing results. Functional validation results showed that overexpression of miR-10a and miR-182 aggravated mitochondrial damage, while inhibition of miR-10a and miR-182 alleviated mitochondrial damage.ConclusionsThis study not only revealed the expression changes of miRNAs and mRNAs in IBRV-infected MDBK cells, but also revealed possible biological regulatory relationship between them. MiR-10a and miR-182 may have the potential to be developed as biomarkers for the diagnosis and treatment of IBRV. Together, Together, these data and analyses provide additional insights into the roles of miRNA and mRNA in IBRV-induced mitochondria damage
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