文献类型： 外文期刊

作者： Sun, Bing ¹ ; Zhao, Xiaohui ¹ ; Gu, Wenxi ¹ ; Cao, Pei ¹ ; Movahedi, Fatemeh ¹ ; Wu, Yanheng ¹ ; Xu, Zhi Ping ¹ ; Gu, Wen ¹ ;

作者机构： 1.Univ Queensland, Australian Inst Bioengn & Nanotechnol, Bldg 75,Corner Cooper Rd & Coll Rd, St Lucia, Qld 4072, Australia

2.Guangzhou Med Univ, GMU GIBH Joint Sch Life Sci, Guangzhou 511436, Peoples R China

3.Xinjiang Acad Anim Sci, Inst Vet Med, Urumqi 830011, Peoples R China

4.Guangzhou Hongkeyuan, Gillion ITM Res Inst, Guangzhou 510530, Peoples R China

期刊名称：JOURNAL OF MATERIALS CHEMISTRY B （ 影响因子：6.331； 五年影响因子：5.726 ）

ISSN： 2050-750X

年卷期： 2021 年 9 卷 36 期

页码：

收录情况： SCI

摘要： Cancer vaccines based on DNA encoding oncogenes have shown great potential in preclinical studies. However, the efficacy of DNA vaccines is limited by their weak immunogenicity because of low cellular internalisation and insufficient activation of dendritic cells (DCs). Calcium phosphate (CP) nanoparticles (NPs) are biodegradable vehicles with low toxicity and high loading capacity of DNA but suffer from stability issues. Here we employed adenosine triphosphate (ATP) as a dual functional agent, i.e. stabiliser for CP and immunological adjuvant, and applied the ATP-modified CP (ACP) NPs to the DNA vaccine. ACP NP-enhanced cellular uptake and improved transfection efficiency of DNA vaccine, and further showed the ability to activate DCs that are critical for them to prime T cells in cancer immunotherapy. As a result, a higher level of antigen-specific antibody with stronger tumour growth inhibition was achieved in mice immunised with the ACP-DNA vaccine. Overall, this one-step synthesised ACP NPs are an efficient nano-delivery system and nano-adjuvant for cancer DNA vaccines.