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Novel putative bluetongue virus serotype 29 isolated from inapparently infected goat in Xinjiang of China

文献类型： 外文期刊

作者： Yang, Heng 1 ; Gu, Wenxi 2 ; Li, Zhanhong 1 ; Zhang, Ling 2 ; Liao, Defang 1 ; Song, Jianling 1 ; Shi, Baoxin 2 ; Hasimu, 1 ;

作者机构： 1.Yunnan Anim Sci & Vet Inst, Yunnan Trop & Subtrop Anim Virus Dis Lab, Kunming, Yunnan, Peoples R China

2.Xinjiang Acad Anim Sci, Inst Vet Med, Urumqi, Xinjiang Autono, Peoples R China

3.Yuli Anim Husb & Vet Stn, Yuli, Xinjiang Autono, Peoples R China

关键词： bluetongue virus; genomic analysis; pathogenicity; putative bluetongue virus serotype 29; virus isolation

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：4.188； 五年影响因子：3.669 ）

ISSN： 1865-1674

年卷期：

页码：

收录情况： SCI

摘要： Bluetongue virus (BTV) is the 'type' species of the genus Orbivirus causing bluetongue (BT) in sheep, bovine and other ruminants. Twenty-four serotypes and several atypical serotypes of BTV were identified worldwide. In present study, a novel strain of BTV (V196/XJ/2014) was isolated from an asymptomatic sentinel goat in Yuli County, Xinjiang of China. Serotype identification of this isolate exhibited uniform negative results by serotype-specific conventional RT-PCR and real-time RT-PCR for BTV-1 to BTV-27, and virus neutralization tests using reference sera of BTV-1 to BTV-24. Genomic analysis showed V196/XJ/2014 grouped with atypical serotypes of BTV-25 to BTV-28, BTV-X/XJ1407, BTV-X/ITL2015 and BTV-Y/TUN2017, while segment 2 and VP2 protein of V196/XJ/2014 shared <63.4%/61.4% nucleic acids and amino acids sequence identities with other recognized BTV serotypes and its segment 2 formed a separate 'nucleotype' in phylogenetic tree. These results indicated V196/XJ/2014 does not belong to any reported serotypes of BTV. Further studies of infectivity and pathogenicity showed that goats infected with V196/XJ/2014 did not exhibit observed clinical symptoms, but high level of virus amplification and homologous neutralization antibodies were detected post-infection. Our studies suggested a novel putative serotype of BTV-29 was isolated in Xinjiang of China, which expands our knowledge about the diversity of BTV.

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