您好，欢迎访问新疆畜牧科学院 机构知识库！

Novel insights into ORFV B2L DNA vaccine-mediated gut microbiota modulation and immune augmentation in rats

文献类型： 外文期刊

作者： Farooq, Umar 1 ; Liu, Guiqiong 1 ; Ahmed, Sohail 1 ; Yang, Huiguo 3 ; Ahmed, Mehboob 1 ; Jiang, Xunping 1 ;

作者机构： 1.Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan, Peoples R China

2.Huazhong Agr Univ, Coll Anim Sci & Technol, Lab Small Ruminant Genet Breeding & Reprod, Wuhan, Peoples R China

3.Xinjiang Acad Anim Sci, Dept Anim Sci Res Inst, Urumqi, Peoples R China

关键词： Orf virus; DNA vaccine; gut microbiota; immune response; vaccine development

期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：5.9； 五年影响因子：6.8 ）

ISSN： 1664-3224

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： The Orf virus (ORFV) poses a significant threat to livestock and human health, causing economic losses in the livestock industry and potential zoonotic infections. Given the limitations of current vaccines, the objective of this study was to investigate the immune response and gut microbiota modulation induced by the ORFV B2L gene-based DNA vaccine (GV) and the live attenuated vaccine (LV) in rats. The findings of this study will provide a scientific foundation for the development of more effective vaccines. Female Sprague-Dawley rats, which were free of specific pathogens, were divided into three groups. The experiment included three groups: the first group was designated as the GV group, the second group was designated as the LV group, and the third group was designated as the control group. Rats in the GV group received intra-muscular injection of 100 mu g/dose of pVAX - B2L - asd plasmid, those in the LV group were immunized with a commercial live - attenuated vaccine, and the control group was injected with PBS. After immunization, various immune - related parameters, such as T - cell subsets, antibody levels, cytokines, and oxidative stress markers, were measured. To this end, composition and function of gut microbiota were thoroughly examined through the implementation of 16S rRNA gene sequencing and PICRUSt-2 functional prediction. The GV group exhibited elevated levels of cellular and humoral immunity. It had a higher percentage of CD4+ and CD8+ T cells, enhanced levels of cytokines i.e. IL - 2, IL - 6, and TNF - alpha, elevated IgA, IgG antibody production compared to the LV and control groups. Additionally, the GV group showed reduced oxidative stress. In terms of gut microbiota, GV immunization led to an increase in beneficial bacteria like Lachnospi-raceae_NK4A136_group and a decrease in harmful or potentially pathogenic bacteria. KEGG pathway analysis indicated that differential flora exhibited an increase in metabolic pathway diversity, including those related to biological systems, metabolism, and human diseases. In sum, the results of the present study demonstrate that the ORFV B2L DNA vaccine (GV) elicited a more robust immune response and exerted a beneficial effect on composition and function of the gut microbiota compared with ORF live-attenuated vaccine. The results of the present study indicate that modulation of gut microbiota by GV vaccine play a crucial role in enhancing vaccine efficacy. The current study provides new perspectives on ORFV vaccine development and its association with vaccines and gut microbiota modulation.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆畜牧科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台