miR-329b-5p Affects Sheep Intestinal Epithelial Cells against Escherichia coli F17 Infection
文献类型： 外文期刊
作者： Xu, Yeling 1 ; Chen, Weihao 1 ; Yang, Huiguo 2 ; Song, Zhenghai 3 ; Wang, Yeqing 4 ; Su, Rui 5 ; Mwacharo, Joram M. 6 ; Lv, Xiaoyang 7 ; Sun, Wei 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China
2.Xinjiang Acad Anim Sci, Inst Anim Husb, Urumqi 830013, Peoples R China
3.Dongshan Anim Epidem Prevent Stn, Suzhou 215100, Peoples R China
4.Suzhou Taihu Dongshang Sheep Ind Dev Co Ltd, Suzhou 215000, Peoples R China
5.Suzhou Stud Farm Co Ltd, Suzhou 215200, Peoples R China
6.Int Ctr Agr Res Dry Areas, Addis Ababa 999047, Ethiopia
7.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety Minist Edu, Yangzhou 225009, Peoples R China
8.Int Joint Res Lab Univ Jiangsu Prov China Domest A, Yangzhou 225009, Peoples R China
关键词： Escherichia coli F17; micro RNA; sheep diarrhea; intestinal epithelial cells
期刊名称：VETERINARY SCIENCES （ 影响因子：2.4； ）
ISSN：
年卷期： 2024 年 11 卷 5 期
页码：
收录情况： SCI
摘要： Sheep diarrhea is one of the most common illnesses in sheep farming and diarrhea in sheep caused by E. coli occupies a significant proportion of them. E. coli F17 is one of the main members of E. coli causing diarrhea in sheep. This study is devoted to improving the resistance of sheep itself to E. coli F17. This study provides theoretical support for the solution of E. coli F17-caused diarrhea in sheep. Diarrhea is the most common issue in sheep farms, typically due to pathogenic Escherichia coli (E. coli) infections, such as E. coli F17. microRNA, a primary type of non-coding RNA, has been shown to be involved in diarrhea caused by pathogenic E. coli. To elucidate the profound mechanisms of miRNA in E. coli F17 infections, methods such as E. coli F17 adhesion assay, colony counting assay, relative quantification of bacterial E. coli fimbriae gene expression, indirect immune fluorescence (IF), Cell Counting Kit-8 (CCK-8), 5-ethynyl-2 '-deoxyuridine (EdU), Western blotting (WB), and scratch assay were conducted to investigate the effect of miR-329b-5p overexpression/knock-down on E. coli F17 susceptibility of sheep intestinal epithelial cells (IECs). The findings indicated that miR-329b-5p enhances the E. coli F17 resistance of sheep IECs to E.coli F17 by promoting adhesion between E. coli F17 and IEC, as well as IEC proliferation and migration. In summary, miR-329b-5p plays a crucial role in the defense of sheep IECs against E. coli F17 infection, providing valuable insights into its mechanism of action.
- 相关文献
作者其他论文 更多>>
-
The Role of BMP7 in the Proliferation of Hu Sheep Dermal Papilla Cells Is Influenced by DNA Methylation
作者：Lv, Xiaoyang;Sun, Wei;Lv, Xiaoyang;He, Mingliang;Wang, Shanhe;Sun, Wei;Lv, Xiaoyang;Wang, Shanhe;Sun, Wei;Zheng, Wenxin;Zhou, Hanlin;Mwacharo, Joram M.
关键词：Hu sheep; lamb skin; BMP7 gene; methylation; dermal papilla cell
-
Effects of yeast culture on in vitro ruminal fermentation and microbial community of high concentrate diet in sheep
作者：Wang, Hongze;Liu, Guiqiong;Zhou, Aimin;Ahmed, Sohail;Farooq, Umar;Wang, Chaoli;Chen, Yan;Ding, Yi;Jiang, Xunping;Wang, Hongze;Kang, Kun;Li, Biao;Yang, Huiguo;Ding, Yi;Jiang, Xunping;Hou, Fuqing;Wang, Chaoli;Bai, Xue;Ding, Yi;Jiang, Xunping;Zhou, Aimin
关键词：Yeast culture; Rumen fermentation; Gas production; Bacterial community; Fungal community
-
Synergistic Effect of Maternal Micronutrient Supplementation on ORFV DNA Vaccine Immune Response in a Pregnant Model
作者：Ahmed, Sohail;Farooq, Umar;Yi, Ding;Sha, Yiyu;Wang, Xiaodong;Ahmed, Mehboob;Jiang, Xunping;Ahmed, Sohail;Liu, Guiqiong;Yi, Ding;Sha, Yiyu;Jiang, Xunping;Sadiq, Amber;Yang, Huiguo;Liu, Yongbin
关键词：Maternal vaccination; ORF virus; Oxidative stress; Pregnancy; Selenium; Vitamin E
-
m6A Methylation Mediates the Function of the circRNA-08436/miR-195/ELOVL6 Axis in Regards to Lipid Metabolism in Dairy Goat Mammary Glands
作者：Wang, Yu;Zhang, Ning;Chen, Zhi;Wang, Yu;Zhang, Ning;Chen, Zhi;Wang, Yu;Wu, Yanni;Yang, Sitian;Gao, Rui;Lv, Xiaoyang;Yang, Zhangping;Chen, Zhi;Lv, Xiaoyang;Yang, Zhangping;Chen, Zhi;Lv, Xiaoyang;Chen, Zhi;Jiao, Peixin;Loor, Juan J.;Loor, Juan J.
关键词：circRNA-08436; miR-195; ELOVL6; M(6)A methylation
-
Biochemical properties of sheep colostrum and its potential benefits for lamb survival: a review
作者：Farooq, Umar;Ahmed, Sohail;Liu, Guiqiong;Jiang, Xunping;Farooq, Umar;Ahmed, Sohail;Liu, Guiqiong;Jiang, Xunping;Yang, Huiguo;Ding, Jianping;Ali, Mehboob
关键词：Sheep; colostrum; productivity; passive immune transfer; lamb survival rate
-
LAD-RCNN: A Powerful Tool for Livestock Face Detection and Normalization
作者：Sun, Ling;Jiang, Xunping;Sun, Ling;Liu, Guiqiong;Jiang, Xunping;Liu, Junrui;Wang, Xu;Yang, Han;Yang, Shiping;Sun, Ling;Liu, Guiqiong;Jiang, Xunping;Yang, Huiguo;Jiang, Xunping
关键词：livestock face detection; rotation angle detection; livestock face normalization; face recognition
-
Selection signature analysis reveals RDH5 performed key function in visionduring sheep domestication process
作者：Hu, Ruixue;Jiang, Xunping;Liu, Guiqiong;Yang, Huiguo;Hu, Ruixue;Jiang, Xunping;Liu, Guiqiong
关键词：
