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m6A Methylation Mediates the Function of the circRNA-08436/miR-195/ELOVL6 Axis in Regards to Lipid Metabolism in Dairy Goat Mammary Glands

文献类型： 外文期刊

作者： Wang, Yu 1 ; Wu, Yanni 3 ; Yang, Sitian 3 ; Gao, Rui 3 ; Lv, Xiaoyang 3 ; Yang, Zhangping 3 ; Jiao, Peixin 6 ; Zhang, Ning 1 ; Loor, Juan J. 7 ; Chen, Zhi 1 ;

作者机构： 1.Xinjiang Acad Anim Sci, Inst Biotechnol, Key Lab Anim Biotechnol Xinjiang, Urumqi 830000, Peoples R China

2.Xinjiang Acad Anim Sci, Inst Biotechnol, Key Lab Genet Breeding & Reprod Grass Feeding Live, Minisitry Agr & Rural Affairs, Urumqi 830000, Peoples R China

3.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China

4.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ, Yangzhou 225009, Peoples R China

5.Int Joint Res Lab Univ Jiangsu Prov China Domest A, Yangzhou 225009, Peoples R China

6.Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China

7.Univ Illinois, Dept Anim Sci, Mammalian Nutr Physiol Genom, Urbana, IL 61801 USA

8.Univ Illinois, Div Nutr Sci, Urbana, IL 61801 USA

关键词： circRNA-08436; miR-195; ELOVL6; M(6)A methylation

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.0 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 12 期

页码：

收录情况： SCI

摘要： The nutritional value of goat milk is determined by the composition of its fatty acids, with particular importance placed on the role of unsaturated fatty acids in promoting human health. CircRNAs have been known to affect fatty acid metabolism through different pathways. In this study, high-throughput sequencing was employed to construct expression profiles of mammary tissue harvested during the dry period and peak lactation stages of dairy goats. Differentially expressed circRNAs and mRNAs were screened, revealing significantly higher expression levels of circRNA-08436 and ELOVL6 during the peak lactation period compared with the dry period. Thus, circRNA-08436 and ELOVL6 were chosen for subsequent studies. The findings demonstrated that circRNA-08436 not only promotes the synthesis of triglyceride (TAG) and cholesterol in goat mammary epithelial cells (GMECs), but also increases the concentrations of saturated fatty acids in the cells. Through the utilization of software prediction, the dual luciferase reporter system, and qRT-PCR, it was observed that circRNA-08436 binds to miR-195, with its overexpression reducing the expression levels of miR-195 and inhibiting TAG synthesis. In addition, circRNA-08436 upregulated the expression levels of the miR-195 target gene ELOVL6. The data also revealed that YTHDC1 facilitated the transport of circRNA-08436 from the nucleus to the cytoplasm, while YTHDC2 in the cytoplasm functioned as a "reader" to identify and degrade circRNA-08436. Taken together, these findings contribute to a better understanding of the molecular regulation of fatty acid metabolism in the mammary glands of dairy goats, thus offering a sound theoretical basis for the production of high-quality goat milk.

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