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Sheep IGFBP2 and IGFBP4 promoter methylation regulates gene expression and hair follicle development

文献类型： 外文期刊

作者： Tian, Yuezhen 1 ; Du, Jianwen 2 ; Yang, Xuemei 2 ; Zeng, Weidan 2 ; He, Junmin 3 ; Zhao, Bingru 4 ; Fu, Xuefeng 1 ; Xu, Xinming 1 ; Wu, Weiwei 1 ; Di, Jiang 1 ; Huang, Xixia 2 ; Tian, Kechuan 1 ;

作者机构： 1.Inst Anim Sci, Xinjiang Acad Anim Sci, Key Lab Genet Breeding & Reprod Xinjiang Cashmere, Urumqi, Peoples R China

2.Xinjiang Agr Univ, Coll Anim Sci, Urumqi, Peoples R China

3.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou, Peoples R China

4.China Agr Univ, Coll Anim Sci & Technol, Beijing, Peoples R China

关键词： DNA methylation; Epigenetic marker; Gene expression; Hair follicle development; IGFBP2; IGFBP4; Promoter; Sheep; Skin; Super Merino; Wool

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.826； 五年影响因子：3.511 ）

ISSN： 0717-3458

年卷期： 2022 年 59 卷

页码：

收录情况： SCI

摘要： Background: Hair follicle development is important for the fineness of wool. Our previous methylated DNA immunoprecipitation sequencing showed that IGFBP2 and IGFBP4 were differentially methylated at different development stages of the hair follicle of Super Merino sheep. This study selected these two genes as candidates to uncover the influence of their promoter methylation on hair follicle development.Results: The results showed that the expression levels of IGFBP2 and IGFBP4 in the skin of very fine wool Super Merino sheep were higher than that in the very thick wool group, and the difference for IGFBP4 was significant. The total methylation rate of IGFBP2 promoter was extremely low, and no significant differ-ence was found in the skin tissue of the Super Merino sheep at different fineness groups. However, the total methylation rate of IGFBP4 promoter in the very thick group was significantly higher than that in the very fine group, and the total DNA methylation levels of the IGFBP4 were significantly negatively cor-related with mRNA expression. Furthermore, the transcription factor prediction results showed 104 and 91 candidate binding sites for transcription factors in the promoter regions of IGFBP2 and IGFBP4, respectively. Among these, JUN, EP300, SP1, PAX6, ETS1, and MYOD1 may be related to hair follicle development.Conclusions: The total methylation level of IGFBP4 promoter was negatively correlated with its mRNA expression in Super Merino sheep skin tissues with different degrees of fineness, which could be used as an epigenetic marker for wool breeding in Super Merino sheep.How to cite: Tian Y, Du J, Yang X, et al. Sheep IGFBP2 and IGFBP4 promoter methylation regulates gene expression and hair follicle development. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2 022.07.001.(c) 2022 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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