Proteomics Reveals the Role of PLIN2 in Regulating the Secondary Hair Follicle Cycle in Cashmere Goats
文献类型： 外文期刊
作者： Wu, Cuiling 1 ; Lu, Qingwei 2 ; Ma, Shengchao 2 ; Mamat, Nuramina 1 ; Tang, Sen 2 ; Liu, Wenna 2 ; Wang, Yaqian 2 ; Anwar, Asma 2 ; Lu, Yingjie 2 ; Ma, Qiangqiang 2 ; Aimaier, Gulinigaer 1 ; Fu, Xuefeng 2 ;
作者机构： 1.Xinjiang Normal Univ, Coll Life Sci, Int Ctr Collaborat Management Cross Border Pest Ce, Xinjiang Key Lab Special Species Conservat & Regul, Urumqi 830054, Peoples R China
2.Xinjiang Acad Anim Sci, Inst Biotechnol, Xinjiang Key Lab Anim Biotechnol, Key Lab Herbivorous Anim Genet Breeding & Reprod,M, Urumqi 830011, Peoples R China
关键词： Jiangnan cashmere goats; secondary hair follicle cycle; proteomics; PLIN2
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 6 期
页码：
收录情况： SCI
摘要： Based on comprehensive proteomic analysis conducted across various stages of secondary hair follicles (SHFs), the growth and development regulatory mechanisms of SHFs in Jiangnan cashmere goats were studied. Proteomic analysis of skin tissue from the SHF anagen (An), catagen (Cn), and telogen (Tn) revealed 145 differentially expressed proteins (DEPs) between the An and Tn, 53 DEPs between the Cn and An, and 168 DEPs between the Cn and Tn. Gene Ontology (GO) annotations indicated that the DEPs were predominantly involved in keratin filament formation (KRTAP3-1, KRT1, KRT8), intermediate filament formation (KRT26, KRT35, KRT19, etc.), and lipid metabolism (FA2H, CERS6, ECH1, TECR, etc.). Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis identified significant enrichment of DEPs in pathways related to hair follicle growth and development. Notably, these included the PPAR signaling pathway (PLIN2, PLIN4, ACSL5, etc.), the IL-17 signaling pathway (S100A7A, LOC108633164), and the estrogen signaling pathway (KRT26, KRT35, LOC102176457.). Western blotting (WB) experiments were then performed on five DEPs (KRT28, FA2H, PLIN2, FABP7, and VNN1) to validate the consistency of the WB results with the proteomic data. Overexpression and siRNA interference of PLIN2 in dermal papilla cells (DPCs) were followed by CCK8 and flow cytometry assays, revealing that PLIN2 knockdown significantly decreased DPC proliferation while inducing apoptosis, compared to controls. These findings suggest that the PLIN2 gene plays a crucial role in modulating SHF growth cycles in cashmere goats by influencing DPC proliferation. These results provide novel insights that could inform the development of breeding strategies aimed at enhancing the cashmere yield in such goats.
- 相关文献
作者其他论文 更多>>
-
The m6A reader IGF2BP3 facilitates myogenesis by activating the CAMK2B-MEF2C axis
作者：Liu, Zhipeng;Deng, Kaiping;Su, Yalong;Wei, Wurilege;Zhang, Zhen;Gao, Yang;Wang, Ziyu;Zhao, Hualin;Fan, Yixuan;Zhang, Guomin;Zhang, Yanli;Wang, Feng;Wei, Wurilege;Liu, Zhipeng;Lu, Qingwei;Lu, Qingwei
关键词：m6A; IGF2BP3; Myogenesis; CAMK2B-MEF2C axis; Post-transcriptional regulation
-
Identification of lncRNAs involved in the hair follicle cycle transition of cashmere goats in response to photoperiod change
作者：Yang, Min;Li, Yingying;Liang, Qianqian;Dong, Huajiao;Han, Jilong;Ma, Yuehui;Andersson, Goeran;Bongcam-Rudloff, Erik;Ahmad, Hafiz Ishfaq;Fu, Xuefeng
关键词：Cashmere goat; LncRNA; Shortened photoperiod; Hair follicle cycle; WGCNA; Transcriptomic analysis
-
A Developmental Gene Expression Atlas Reveals Novel Biological Basis of Complex Phenotypes in Sheep
作者：Zhao, Bingru;Zhang, Guoming;Wang, Feng;Zhang, Yanli;Luo, Hanpeng;Fu, Xuefeng;Zhang, Guoming;Clark, Emily L.;Dalrymple, Brian Paul;Xiang, Ruidong;Dalrymple, Brian Paul;Vercoe, Philip E.;Dalrymple, Brian Paul;Vercoe, Philip E.;Oddy, V. Hutton;Wu, Cuiling;Liu, George E.;Li, Cong-jun;Xiang, Ruidong;Xiang, Ruidong;Xiang, Ruidong;Tian, Kechuan;Fang, Lingzhao
关键词：Sheep; Developmental biology; Gene Expression Atlas; Genome-wide association study; Complex trait
-
Genetic Evaluation of Early Growth Traits in Yunnan Semi-Fine Wool Sheep
作者：Wang, Yaqian;Lu, Qingwei;Wang, Yaqian;Lu, Qingwei;Tang, Sen;Fu, Xuefeng;Yang, Hongyuan;Zhao, Xiaoqi;Ni, Xiaojun;Quan, Guobo;Zhao, Yuanchong;You, Zhengrong
关键词：Yunnan semi-fine wool sheep; genetic parameters; early growth traits
-
Metabolomics reveals metabolites associated with hair follicle cycle in cashmere goats
作者：Ma, Shengchao;Cao, Wenzhi;Ma, Xiaolin;Ye, Xiaofang;Wu, Cuiling;Ma, Shengchao;Cao, Wenzhi;Ma, Xiaolin;Ye, Xiaofang;Wu, Cuiling;Ma, Shengchao;Liu, Wenna;Lu, Qingwei;Fu, Xuefeng;Qin, Chongkai;Li, Bin
关键词：Cashmere goat; Hair follicle cycle; Metabolome of skin tissue; Relationships; Influencing factors
-
Transcriptome meta-analysis reveals the hair genetic rules in six animal breeds and genes associated with wool fineness
作者：Pu, Xue;Cen, Yunlin;Wu, Cuiling;Pu, Xue;Ma, Shengchao;Zhao, Bingru;Lu, Qingwei;Liu, Wenna;Wang, Yaqian;Fu, Xuefeng;Ma, Shengchao;Lu, Qingwei;Liu, Wenna;Wang, Yaqian;Zhao, Bingru;Tang, Sen
关键词：Ordos fine wool sheep; six livestock breeds; wool fineness; meta-analysis; WGCNA
-
Comparison of different animal models for estimating genetic parameters for early growth traits and reproductive traits in Tianmu Sainuo sheep
作者：Liu, Wenna;Lu, Qingwei;Wang, Yaqian;Liu, Wenna;Lu, Qingwei;Pu, Xue;Wang, Yaqian;Hu, Xiangrong;Fu, Xuefeng;Tang, Sen;Pu, Xue;Wu, Cuiling;Hong, Wei
关键词：sheep; litter size; model evaluation; non-genetic factors; fixed effects; heritability
