文献类型： 外文期刊

作者： Hu, Xi-Dan ² ; Yu, Da-Hai ¹ ; Chen, Su-Ting ¹ ; Li, Shu-Xia ² ; Cai, Hong ¹ ;

作者机构： 1.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China

2.Xinjiang Acad Anim Sci, Inst Vet Med, Urumqi, Peoples R China

关键词： Animals;Brucella abortus genetics immunology;Brucellosis;Bovine immunology prevention & control;Cattle;Enzyme-Linked Immunosorbent Assay;Interferon-gamma immunology;Lung pathology;Male;Mice;Mycobacterium bovis genetics immunology;Peptide Fragments immunology;Tuberculosis Vaccines immunology;Tuberculosis;Bovine immunology prevention & control;Vaccines;Combined immunology;Vaccines;DNA immunology

期刊名称：DNA AND CELL BIOLOGY （ 影响因子：3.311； 五年影响因子：3.557 ）

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收录情况： SCI

摘要： We evaluated the immunogenicity and protective efficacy of a combined DNA vaccine containing six genes encoding immunodominant antigens from Mycobacterium bovis and Brucella abortus. The number of lymph node and spleen cultures positive for M. bovis and B. abortus from calves immunized with the combined DNA vaccine was significantly reduced (p < 0.01) compared with unvaccinated calves after challenge with virulent M. bovis and B. abortus 544. The combined DNA vaccine group displayed stronger antigen-specific interferon-gamma (IFN-gamma) responses and antigen-specific IFN-gamma ELISPOT activities 2 months after final immunization and after challenge. Antigen-specific CD4(+) and CD8(+) T cell responses in the combined DNA vaccine group were higher than either the Bacillus Calmette-Guerin (BCG)-positive or S19-positive control group. Likewise, more calves in the DNA vaccine group exhibited antigen-specific IgG titers and had higher IgG titers than those in the BCG- or S19-immunized groups 2 months after the final immunization. Moreover, two antigens in the combined DNA vaccine induced significant antigen-specific IFN-gamma responses 6 months after challenge (p < 0.05). Bacterial counts and pathological analyses of the challenged animals indicated that the combined DNA vaccine provided significantly better protection than the BCG vaccine against M. bovis, and the protection level induced by the combined DNA vaccine was comparable to S19 against B. abortus. This is the first report to demonstrate that a single combined DNA vaccine protects cattle against two infectious diseases.