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Identification of a conserved linear epitope using monoclonal antibody against non-structural protein 3A of foot-and-mouth disease virus with potential for differentiation between infected and vaccinated animals

文献类型： 外文期刊

作者： Wang, Mingxia 1 ; Xu, Zhiqiang 1 ; Liu, Wenming 1 ; Li, Minjie 1 ; Wang, Haiwei 1 ; Yang, Decheng 1 ; Ma, Wenge 2 ; Zhou, 1 ;

作者机构： 1.Chinese Acad Agr Sci, Div Livestock Infect Dis, State Key Lab Vet Biotechnol, Harbin Vet Res Inst, 678 Haping Rd Xiangfang Dist, Harbin 150069, Heilongjiang, Peoples R China

2.Xinjiang Acad Anim Sci, Inst Vet Med, 151 Eastern Kelamayi St, Urumqi 830000, Peoples R China

关键词： Foot-and-mouth disease virus; Non-structural protein 3A; Monoclonal antibody; Epitope mapping

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN： 0034-5288

年卷期： 2019 年 124 卷

页码：

收录情况： SCI

摘要： Foot-and-mouth disease (FMD) is a highly contagious and economically devastating viral disease of cloven-hoofed animals. Vaccination is a key element in the control of FMD among countries where the disease is enzootic. Differentiating infected from vaccinated animals in herds after immunization is an important component of effective eradication strategies. Non-structural protein (NSP) 3A of FMDV is as part of a larger detected antigen that is used for this differential diagnosis. Here, we generated a specific monoclonal antibody (MAb) against FMDV non-structural protein called 3A10, and further defined the linear epitopes recognized by the MAb 3A10 using a series of peptides that expressed GST-fused protein. Using Western blot, it was showed that the 5-aa peptide (ERTLP130)-E-126 of 3A was the minimal epitope reactive to MAb 3A10. Alanine-scanning mutagenesis analysis revealed that Arg127 and Leu129 were crucial for MAb 3A10 binding to (ERTLP130)-E-126. Furthermore, sequence alignment analysis, indicated that the epitope (ERTLP130)-E-126 recognized by 3A10 was shown to be conserved among seven serotypes of FMDV strains. The synthetic peptide Elisa demonstrated that this epitope peptide could be recognized by sera from FMDV-infected pigs and cattle, but negative reactivity to unvaccinated and vaccinated healthy animal sera. Thus, the MAb reagents and the linear epitopes defined herein provide theoretical and technical support for the development of diagnostic tools for infection differentiating FMDV infected from vaccinated animals.

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