文献类型： 外文期刊

作者： Liu, Wenming ¹ ; Yang, Baolin ¹ ; Wang, Mingxia ¹ ; Wang, Haiwei ¹ ; Yang, Decheng ¹ ; Ma, Wenge; Zhou, Guohui ¹ ; Yu, ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Div Livestock Infect Dis, 678 Haping Rd, Harbin 150069, Heilongjiang, Peoples R China

2.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Bio

关键词： Foot-and-mouth disease virus;Neutralizing epitope;Monoclonal antibody;Phage display

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

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收录情况： SCI

摘要： AbstractFoot-and-mouth disease (FMD) caused by foot-and-mouth disease virus (FMDV), is a highly contagious infectious disease that affects domestic and wild cloven-hoofed animals worldwide. In recent years, outbreaks of serotype A FMD have occurred in many countries. High-affinity neutralizing antibodies against a conserved epitope could provide protective immunity against diverse subtypes of FMDV serotype A and protect against future pandemics. In this study, we generated a serotype A FMDV-specific potent neutralizing monoclonal antibody (MAb), 6C9, which recognizes a conformation-dependent epitope. MAb 6C9 potently neutralized FMDV A/XJBC/CHA/2010 with a 50% neutralization titer (NT50) of 4096. Screening of a phage-displayed random 12-mer peptide library revealed that MAb 6C9 bound to phages displaying the consensus motif YxxPxGDLG, which is highly homologous to the135YxxPxxxxxGDLG147motif found in the serotype A FMDV virus-encoded structural protein VP1. To further verify the authentic epitope recognized by MAb 6C9, two FMDV A/XJBC/CHA/2010 mutant viruses, P138A and G144A, were generated using a reverse genetic system. Subsequent micro-neutralization assays and double-antibody sandwich (DAS) ELISA analyses revealed that the Pro138and Gly144residues of the conformational epitope that are recognized by 6C9 are important for MAb 6C9 binding. Importantly, the epitope135YxxPxxxxxGDLG147was highly conserved among different topotypes of serotype A FMDV strains in a sequence alignment analysis. Thus, the results of this study could have potential applications in the development of novel epitope-based vaccines and suitable a MAb-based diagnostic method for the detection of serotype A FMDV and the quantitation of antibodies against this serotype.Highlights?Generated a serotype A FMDV-specific potent neutralizing MAb, 6C9?The MAb 6C9 bound to phages displaying the consensus motif YxxPxGDLG?The residues Pro138and Gly144of the conformational epitope are important for MAb 6C9 binding?Potential applications in vaccines and diagnostic method]]>