文献类型： 外文期刊

作者： Qi, Meng ¹ ; Wang, Haiyan ³ ; Jing, Bo ⁴ ; Wang, Dengfeng ⁵ ; Wang, Rongjun ¹ ; Zhang, Longxian ¹ ;

作者机构： 1.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

2.Int Joint Res Lab Zoonot Dis Henan, Zhengzhou 450002, Peoples R China

3.Henan Vocat Coll Agr, Dept Anim Sci, Zhongmu 451450, Peoples R China

4.Tarim Univ, Coll Anim Sci, Alar 843300, Peoples R China

5.Xinjiang Acad Anim Sci, Inst Vet Med, Urumqi 830000, Peoples R China

关键词： Cryptosporidium;Dairy calves;Xinjiang;Prevalence;Identification

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

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收录情况： SCI

摘要： A total of 514 fecal samples were collected from dairy calves on 15 farms in the Xinjiang Uyghur Autonomous Region in Northwestern China and were screened for Gyptosporidium spp. by PCR. The overall prevalence of Cryptosporidium was 16.0% (82/514), and the infection rate was 15.6% (37/237) and 16.2% (45/277) in pre- and post-weaned calves, respectively. PCR-restriction fragment length polymorphism (RFLP) and DNA sequence analyses of 82 positive fecal samples revealed the presence of four Cryprosporidium species; of these, 22 were Ctyptosporidium parvum, 20 were Corynebacterium bovis, 9 were Cryptosporidium ryanae, 25 were Cryptosporidium andersoni, 2 were mixed C. bovis/C. parvum, and 4 were mixed C. bovis/C. ryanae infections. In pre-weaned calves, C. parvum was the most common species (22/37, 59.5%). In contrast, C andersoni was the dominant species (23/45, 51.1%) in post-weaned calves. Subtyping analysis based on the 60-kDa glycoprotein (gp60) gene successfully identified 15 C. parvum isolates as being in the lid family; 11 were IIdA15G1 and 4 were IIdA14G1. Recent findings describing the C. parvum IId subtypes as the dominant group in humans and animals in China indicate that dairy calves may be an important source of zoonotic Cryptosporidium in China. (C) 2015 Elsevier B.V. All rights reserved.