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Cryopreservation of stallion spermatozoa using different cryoprotectants and combinations of cryoprotectants

文献类型： 外文期刊

作者： Wu, Zhuangyuan 1 ; Zheng, Xinbiao 2 ; Luo, Yongming 2 ; Huo, Fei 2 ; Dong, Hong 2 ; Zhang, Guoting 2 ; Yu, Weihao 3 ; Tia 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832000, Xinjiang, Peoples R China

2.Xinjiang Acad Anim Sci, Inst Anim Sci, Urumqi 830000, Xinjiang, Peoples R China

3.Breeding Ctr Yili Horse, Zhaosu 835600, Xingjiang, Peoples R China

关键词： Frozen semen;Equine spermatozoa;Horse;Cryoprotective agents;CPA

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

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收录情况： SCI

摘要： The present study investigates the effects of five cryoprotectants (CPAs) and cryoprotectant combinations on the post-thaw total motility, progressive motility, viability, mitochondrial membrane potential and acrosome integrity in stallion spermatozoa. In Experiment 1, the objective was to compare the impact of different concentrations (2.5%, 3.5% and 5%) of a single CPA, including glycerol (Gly), ethylene glycol (EG), dimethyl sulphoxide (DMSO), methyl formamide (MF), and dimethylformamide (DMF) for stallion spermatozoa cryopreservation. In Experiment 2, two or more CPAs were used to assess whether this improved post-thaw spermatozoa quality. Gly, MF and DMF, were used to prepare seven combinations of freezing extender with different mixtures of cryoprotectant, and the 3.5% Gly, MF and DMF were used as a control group. The results show that post-thaw total motility, progressive motility, viability, and mitochondrial membrane potential for all concentrations of EG and DMSO were less than the 3.5% and 5% Gly and MF and 2.5% and 3.5% DMF (P<0.05). Use of the 3.5% concentration resulted in the greater post-thaw total motility and progressive motility than the 2.5% and 5% concentrations for all CPAs. The results for the use of different combinations of cryoprotectant indicate there are differences in progressive motility and viability. The viability with the use of Gly(2/3)+ MF(1/3) was 44.65% and was greater than the Gly(1/3) + MF(1/3)+ DMF(1/3) (30.96%), MF(2/3) + DMF(1/3) (35.05%), Gly (32.21%) and MF(33.76%) (P<0.05). The progressive motility with the use of the MF(2/3)+ Gly(1/3) combination was 36.0% and was greater than in the DMF (25.0%) and MF(2/3)+ DMF(1/3) (22.7%) (P<0.05). These results suggest that using the appropriate cryoprotectant combination instead of a single cryoprotectant can improve horse spermatozoa cryopreservation. (C) 2015 Elsevier B.V. All rights reserved.

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