Effect of the FA2H Gene on cashmere fineness of Jiangnan cashmere goats based on transcriptome sequencing
文献类型： 外文期刊
作者： Wu, Cuiling 1 ; Li, Jianying 4 ; Xu, Xinming 3 ; Xu, Qi 4 ; Qin, Chongkai 5 ; Liu, Guifen 1 ; Wei, Chen 1 ; Zhang, Guoping 2 ; Tian, Kechuan 1 ; Fu, Xuefeng 3 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Shandong, Peoples R China
2.Xinjiang Agr Univ, Coll Anim Sci, Urumqi 830052, Peoples R China
3.Xinjiang Acad Anim Sci, Inst Anim Sci, Key Lab Genet Breeding & Reprod Xinjiang Wool She, Urumqi 830011, Peoples R China
4.Xinjiang Mil Gen Hosp, Key Lab Special Environm Med, Urumqi 830000, Peoples R China
5.Xinjiang Aksu Prefecture Anim Husb Technol Extens, Aksu 843000, Peoples R China
关键词： Jiangnan cashmere goats; Cashmere fineness; FA2H; Dermal papilla cells
期刊名称：BMC GENOMICS （ 影响因子：4.547； 五年影响因子：4.931 ）
ISSN： 1471-2164
年卷期： 2022 年 23 卷 1 期
页码：
收录情况： SCI
摘要： Background Cashmere goats are a heterogeneous hairy mammal. The fineness of cashmere can affect its economic value. Therefore, in this study, we used transcriptome sequencing techniques to analyze the gene expression profiles of the skin tissues of cashmere goats with different cashmere fineness. The selected candidate genes were functionally verified with the secondary hair follicle hair papillary cells of cashmere goats. Results We identified 479 DEGs, of which 238 mRNAs were up-regulated in the fine velvet group and 241 mRNA were down-regulated. Based on functional annotation and protein interaction network analysis, we found some genes that may affect the fineness of cashmere, including SOX18, SOX4, WNT5A, IGFBP4, KAP8, KRT36, and FA2H. Using qRT-PCR, Western blot, CCK-8 cell viability detection, EDU cell proliferation detection, and flow cytometry, we found that overexpression of the FA2H gene could promote the proliferation of secondary hair follicle DPCs in cashmere goats. At the same time, we proved that FA2H could regulate the expression levels of the FGF5 and BMP2 genes in DPCs. Conclusion The results of this study provide a useful reference for the genetics and breeding of Jiangnan cashmere goats and goat genome annotation, and provide an experimental basis for improving cashmere quality of the cashmere goat.
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