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Differences of the anti-oxidative capability, GPX3, and Cu/ZnSOD expression in Hu sheep testis with different size at six-month-old

文献类型： 外文期刊

作者： Yao, Ting 1 ; Weng, Xiuxiu 1 ; Liang, Weili 1 ; Li, Wanhong 1 ; Wu, Weiwei 2 ; Li, Fadi 1 ;

作者机构： 1.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Engn Res Ctr Grassland Ind, Key Lab Grassland Livestock Ind Innovat,Minist Agr, Lanzhou, Peoples R China

2.Inst Anim Sci, Xinjiang Acad Anim Sci, Key Lab Genet Breeding & Reprod Xinjiang Wool Shee, Urumqi, Peoples R China

3.Gansu Runmu Biol Engn Co Ltd, Yongchang, Peoples R China

关键词： Hu sheep; anti-oxidative capabilities; seminiferous tubule; mtDNA; ROS

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：3.7； 五年影响因子：3.3 ）

ISSN： 1049-5398

年卷期： 2023 年

页码：

收录情况： SCI

摘要： This study aimed to investigate the differences in the anti-oxidant capabilities and related gene expressions of six-month-old Hu sheep with different testis sizes. A total of 201 Hu ram lambs were fed up to 6 months in the same environment. Based on their testis weight and sperm count, 18 individuals were selected and divided into large (n = 9) and small (n = 9) groups, with an average testis weight of 158.67 g +/- 5.21 g and 44.58 g +/- 4.14 g, respectively. The total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and malondialdehyde (MDA) concentration in testis tissue were tested. The localization of antioxidant-related genes, GPX3 and Cu/ZnSOD in testis were detected by immunohistochemistry. The GPX3, Cu/ZnSOD expression, and relative mitochondrial DNA (mtDNA) copy number were detected by quantitative real-time PCR. Compared with the small group, the T-AOC (2.69 +/- 0.47 vs. 1.16 +/- 0.22 U/mgprot) and T-SOD (22.35 +/- 2.59 vs. 9.92 +/- 1.62 U/mgprot) in the large group were significantly higher, whereas the MDA (0.72 +/- 0.13 vs. 1.34 +/- 0.17 nM/mgprot) and relative mtDNA copy number in the large group was significantly lower (p < .05). Immunohistochemistry results indicated that the GPX3 and Cu/ZnSOD were expressed in Leydig cells and seminiferous tubule. The expressions of GPX3 and Cu/ZnSOD mRNA in the large group were significantly higher than those in the small group (p < .05). In conclusion, Cu/ZnSOD and GPX3 widely expressed in the Leydig cells and seminiferous tubule, high expression of Cu/ZnSOD and GPX3 in a large group has a higher potential in addressing oxidative stress and contribute to spermatogenesis.

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