文献类型： 外文期刊

作者： Li, Na ¹ ; Li, Hong-Bo ¹ ; Yan, Xiang-Ming ¹ ; Du, Wei ¹ ; Zhang, Jin-Shan ¹ ; Yuan, Li-Xing ¹ ; Zhang, Yang ¹ ; Yao, Gan ¹ ;

作者机构： 1.Xinjiang Acad Anim Sci, Inst Res Livertock, Urumqi 830000, Xinjiang, Peoples R China

2.Xinjiang Agr Univ, Coll Vet Med, 311 Agr East Rd, Urumqi 830000, Xinjiang, Peoples R China

关键词： Xinjiang brown cattle;different positions;genes regulating lipid metabolism;adipocyte area;adipocyte number per unit area

期刊名称：INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE （ 影响因子：0.166； 五年影响因子：0.621 ）

ISSN： 1940-5901

年卷期： 2016 年 9 卷 3 期

页码：

收录情况： SCI

摘要： Objective: Thirty adult healthy Xinjiang brown cattle were selected and fasted for 12 h before slaughtering. Perirenal fat, subcutaneous fat, pericardial fat and intermuscular fat were collected. Fluorescence quantitative PCR was performed to detect the expressions of leptin gene (lep), fatty acid synthetase gene (fas), lipoprotein lipase gene (lpl) and obesity-associated gene (fto). Cryosectioning technique was employed to prepare the sections of intermuscular fat, subcutaneous fat, perirenal fat and pericardial fat. Adipocyte area and adipocyte number per unit area were calculated using Motic microscopic imaging system. Correlation analysis was carried out with the expression of the four genes regulating lipid metabolism. Results showed that the expressions of the four fat metabolism genes in Xinjiang brown cattle were specific to tissues. Only fas gene did not show significant differential expression. The lep and lpl genes were strongly expressed in intermuscular fat and pericardial fat; fto gene was most strongly expressed in pericardial fat. Four genes showed different expressions in the same adipose tissues. In intermuscular fat, the expression of lep gene was obviously higher than that of other genes; in subcutaneous fat, the expression of fas gene was significantly higher than that of other genes (P<0.05); in perirenal fat, lpl and fto genes were significantly expressed (P<0.05); in pericardial fat, the expression of fto gene was considerably higher than that of other genes. Adipocyte area and adipocyte number were specific to tissues, and in the decreasing order of adipocyte area, adipose tissues were ranked as follows: perirenal fat, subcutaneous fat and pericardial fat, intermuscular fat; in the decreasing order of adipocyte number per unit area, adipose tissues were ranked as follows: intermuscular fat and subcutaneous fat, pericardial fat, perirenal fat. Except fas and fto genes, intermuscular adipocyte area was in significant negative correlation with lep gene expression (r=-0.3656; P<0.05); intermuscular adipocyte area was also correlated with lpl expression (r=-0.51; P=0.0581). Pericardial adipocyte area was significantly negatively correlated with the expressions of lep and lpl genes (r=-0.4133, r=-0.47; P<0.05). Perirenal adipocyte area did not show significant correlations with the expression of any of the four genes (P>0.05). The expression of four genes regulating lipid metabolism was not significantly correlated with the corresponding adipocyte number (P<0.05).