The Echinococcus granulosus Antigen B Gene Family Comprises at Least 10 Unique Genes in Five Subclasses Which Are Differentially Expressed
文献类型： 外文期刊
作者： Zhang, Wenbao 1 ; Li, Jun 1 ; Jones, Malcolm K. 1 ; Zhang, Zhuangzhi 2 ; Zhao, Li 2 ; Blair, David 4 ; McManus, Donald 1 ;
作者机构： 1.Queensland Inst Med Res, Div Infect Dis, Mol Parasitol Lab, Brisbane, Qld 4006, Australia
2.Xinjiang Acad Anim Sci, Xinjiang Vet Res Inst, Urumqi, Xinjiang, Peoples R China
3.Univ Queensland, Sch Vet Sci, Brisbane, Qld, Australia
4.James Cook Univ, Sch Marine & Trop Biol, Townsville, Qld 4811, Australia
期刊名称：PLOS NEGLECTED TROPICAL DISEASES （ 影响因子：4.411； 五年影响因子：4.898 ）
ISSN： 1935-2727
年卷期： 2010 年 4 卷 8 期
页码：
收录情况： SCI
摘要： Background: Antigen B (EgAgB) is a major protein produced by the metacestode cyst of Echinococcus granulosus, the causative agent of cystic hydatid disease. This protein has been shown to play an important role in modulating host immune responses, although its precise biological function still remains unknown. It is generally accepted that EgAgB is comprised of a gene family of five subfamilies which are highly polymorphic, but the actual number of genes present is unknown. Methodology/Principal Findings: Based on published sequences for the family, we designed specific primers for each subfamily and used PCR to amplify them from genomic DNA isolated from individual mature adult worms (MAW) taken from an experimentally infected dog in China and individual larval protoscoleces (PSC) excised from a single hydatid cyst taken from an Australian kangaroo. We then used real-time PCR to measure expression of each of the genes comprising the five EgAgB subfamilies in all life-cycle stages including the oncosphere (ONC). Conclusions/Significance: Based on sequence alignment analysis, we found that the EgAgB gene family comprises at least ten unique genes. Each of the genes was identical in both larval and adult E. granulosus isolates collected from two geographical areas (different continents). DNA alignment comparisons with EgAgB sequences deposited in GenBank databases showed that each gene in the gene family is highly conserved within E. granulosus, which contradicts previous studies claiming significant variation and polymorphism in EgAgB. Quantitative PCR analysis revealed that the genes were differentially expressed in different life-cycle stages of E. granulosus with EgAgB3 expressed predominantly in all stages. These findings are fundamental for determining the expression and the biological function of antigen B.
- 相关文献
作者其他论文 更多>>
-
Ultrasonography screening of hepatic cystic echinococcosis in sheep flocks used for evaluating control progress in a remote mountain area of Hejing County, Xinjiang
作者：Qi, Xinwei;Song, Tao;Li, Zhao;Dong, Jian;Dawuti, Wubulitalifu;Dou, Jingrui;Li, Jun;Wen, Hao;Zhang, Wenbao;Zhang, Zhuangzhi;Jiang, Tao;Wu, Chuanchuan;Zhang, Wenbao;Guo, Gang
关键词：Ultrasonography; Cystic echinococcosis; Echinococcus granulosus; Sheep flocks; Active cysts
-
Investigation and genetic polymorphism analysis of rodents infected with Echinococcus in Ili Prefecture, Xinjiang Uygur Autonomous Region, China
作者：Wang, Bingjie;Zhao, Li;Ban, Wanli;Zhang, Zhuangzhi;Zhang, Xu;Quan, Chenxi;Teliewuhan, Munila;He, Lixiong;Chen, Zhaoyang
关键词：Echinococcus multilocularis; nad1 gene; genetic polymorphism; rodent; Ili
-
The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang
作者：Guo, Baoping;Cairen;Ren, Yuan;Wen, Hao;Wu, Jianyong;Wang, Wenli;Wang, Bingjie;Aizezi, Mayire;Zhao, Li;Mi, Rongsheng
关键词：Alveolar echinococcosis; Echinococcus multilocularis; Yili prefecture; Mitochondrial; Haplotypes; Pairwise fixation index; Variant
-
First report of Echinococcus granulosus genotype 1 in a wild boar (Sus scrofa) from China
作者：Guo, Baoping;Aimulajiang, Kalibixiati;Yimingjiang, Maiweilidan;Wen, Hao;Cairen;Zhao, Li;Tang, Wenqiang;Wu, Chuanchuan;Wu, Jianyong;Mi, Rongsheng
关键词：Cystic echinococcosis; Wild boar; Zhaosu County; Yili Prefecture; Mitochondrial analysis; Histopathological staining; Genotype
-
A Diverse Virome Is Identified in Parasitic Flatworms of Domestic Animals in Xinjiang, China
作者：Zhang, Peng;Cao, Le;Zhang, Chiyu;Li, Yanpeng;Zhang, Yao;Li, Jun;Wu, Chuanchuan;Tian, Mengxiao;Zhang, Wenbao;Zhang, Zhuangzhi;Zhang, Wenbao
关键词：virome; parasitic flatworms; virus evolution; viral metagenomics; metagenomics; virus discovery
-
Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, China
作者：Guo, Baoping;Guo, Gang;Ren, Yuan;Qi, Wenjing;Zhao, Li;Zhang, Xu;Wang, Bingjie;Zhang, Zhuangzhi;Zhao, Lu;Mi, Rongsheng
关键词：Echinococcus granulosus sensu stricto; prevalence; genetic diversity; cox1; Altai
-
High endemicity of alveolar echinococcosis in Yili Prefecture, Xingjiang Autonomous Region, the People's Republic of China: Infection status in different ethnic communities and in small mammals
作者：Guo, Baoping;Guo, Gang;Zheng, Xueting;Bao, Jianling;He, Li;Wang, Tian;Qi, Wenjing;Tian, Mengxiao;Zhou, Canlin;Zhang, Wenbao;Li, Jun;Guo, Baoping;Guo, Gang;Zheng, Xueting;Bao, Jianling;He, Li;Wang, Tian;Qi, Wenjing;Tian, Mengxiao;Zhou, Canlin;Zhang, Wenbao;Li, Jun;Zhang, Zhuangzhi;Wang, Junwei;Guo, Yongzhong;Wang, Haiyan;Ma, Jianjun;Chen, Ronggui;Giraudoux, Patrick;Giraudoux, Patrick;Marston, Christopher G.;McManus, Donald P.
关键词：
