文献类型： 外文期刊
作者： Ye, Xiao-Fang 1 ; Chen, Shi-Bin 1 ; Wang, Li-Qin 1 ; Zhao, Yun-Cheng 1 ; Lv, Xue-Feng 1 ; Liu, Ming-Jun 3 ; Huang, Jun 1 ;
作者机构： 1.Xinjiang Acad Anim Sci, Xinjiang Key Lab Anim Biotechnol, Urumqi 830000, Peoples R China
2.Xinjiang Normal Univ, Coll Life Sci, Urumqi 830000, Peoples R China
3.Xinjiang Acad Anim Sci, Minist Agr, Key Lab Reprod & Breeding Biotechnol Grass Feedin, Urumqi 830000, Peoples R China
关键词： aged oocyte;development;glutathione
期刊名称：REPRODUCTION FERTILITY AND DEVELOPMENT （ 影响因子：2.311； 五年影响因子：2.396 ）
ISSN： 1031-3613
年卷期： 2010 年 22 卷 8 期
页码：
收录情况： SCI
摘要： The intracellular glutathione levels and developmental competence of aged oocytes after parthenogenetic activation, somatic cell nuclear transfer and intracytoplasmic sperm injection in the presence or absence of caffeine or dithiothreitol (DTT) were examined. The following results were found: (1) ovine oocytes were fully aged 30 h post-onset of maturation culture; (2) the appropriate concentrations of caffeine and DTT for oocyte culture were 5 mM and 1 mM, respectively; (3) when nuclear transfer-reconstructed embryos were treated with caffeine or DTT following fusion, no increase in the frequency of development to blastocyst was observed (P > 0.05), but the cell numbers of blastocysts increased (P < 0.05); (4) both caffeine and DTT increased the blastocyst formation rates of intracytoplasmic sperm-injected embryos (P < 0.05); (5) caffeine increased the glutathione content of aged oocytes (P < 0.05). The glutathione content of DTT-treated aged oocytes was higher than that of oocytes matured for 36 h (P < 0.05). In conclusion, caffeine and dithiothreitol delay oocyte ageing but only to a limited extent.
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