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Rapid development of attenuated IBV vaccine candidates through a versatile backbone applicable to variants

文献类型： 外文期刊

作者： Lu, Yuanlu 1 ; Zeng, Yiran 1 ; Luo, Haowei 1 ; Chen, Na 1 ; Zhao, Lingcai 1 ; Zhang, Haitao 2 ; Xia, Jun 3 ; Ping, Jihui 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Engn Lab Anim Immun Jiangsu Prov, Nanjing 210095, Peoples R China

2.Jiangsu Lihua Anim Husb Co Ltd, Biotechnol Res Lab, Changzhou 213168, Peoples R China

3.Minist Agr & Rural Affairs, Xinjiang Acad Anim Sci Inst Vet Med, Key Lab Prevent & Control Herbivorous Anim Dis, Urumqi 830000, Peoples R China

4.Xinjiang Anim Dis Res Key Lab, Urumqi 830000, Peoples R China

期刊名称：NPJ VACCINES （ 影响因子：6.5； 五年影响因子：6.6 ）

ISSN：

年卷期： 2025 年 10 卷 1 期

页码：

收录情况： SCI

摘要： The antigen variability of the infectious bronchitis virus (IBV) has hindered vaccine effectiveness and perpetuated its epidemic. We engineered a rapid attenuation method for IBV variants. The strategy involves creating the rH-CPDF7 backbone by recoding a segment of the H120 nonstructural protein (NSP) genome via codon pair deoptimization (CPD), facilitating S gene integration from IBV variants via transformation-associated recombination (TAR) cloning. These recombinant strains exhibited even lower pathogenicity, indicating the effectiveness of CPDF7 in reducing virulence. Importantly, the rH-CPDF7 backbone demonstrated versatility, being applicable to the development of attenuated strains for IBV variants, including the QX-type, TW-type, and GVI-type strains (different genotypes). In conclusion, our method allows for the rapid development of attenuated strains by integrating the S gene of IBV variants into the rH-CPDF7 backbone. These recombinant strains can elicit a strong immune response and provide effective protection against homologous challenges. This strategy is crucial for developing live-attenuated vaccines against emerging IBV strains.

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