文献类型： 外文期刊

作者： Lu, Yuanlu ¹ ; Zeng, Yiran ¹ ; Luo, Haowei ¹ ; Qiao, Bingchen ¹ ; Meng, Qi ¹ ; Dai, Zijian ¹ ; Chen, Na ¹ ; Zhao, Lingcai ¹ ; Meng, Xianchen ² ; Zhang, Haitao ² ; Xia, Jun ³ ; Ping, Jihui ¹ ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Engn Lab Anim Immun Jiangsu Prov, Nanjing 210095, Peoples R China

2.Lihua Nanjing Ind Res Inst Co Ltd, Nanjing 213168, Peoples R China

3.Xinjiang Acad Anim Sci, Inst Vet Med, Key Lab Prevent & Control Herbivorous Anim Dis, Minist Agr & Rural Affairs, Urumqi 830000, Peoples R China

4.Xinjiang Acad Anim Sci, Inst Vet Med, Xinjiang Anim Dis Res Key Lab, Urumqi 830000, Peoples R China

5.Yangzhou Univ, Coll Vet Med, Key Lab Jiangsu Prevent Vet Med, Minist Educ,Key Lab Avian Prevent Med, Yangzhou 225009, Jiangsu, Peoples R China

关键词： Infectious bronchitis virus; genome recombination; pathogenicity; neutralizing epitope; antigenic variation

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 12 期

页码：

收录情况： SCI

摘要： Infectious bronchitis virus (IBV) is oneof the major avian pathogens plaguing the global poul-try industry. Although vaccination is the primary pre-ventive measure for IBV infection, the emergence ofvirus variants with mutations and recombination hasresulted in IBV circulating globally, presenting a chal-lenge for IB control. Here, we isolated 3 IBV strains(CZ200515, CZ210840, and CZ211063) from suspectedsick chickens vaccinated with IBV live attenuated vac-cines (H120, 4/91, or QXL87). Phylogenetic analysis ofthe S1 gene sequence of the spike (S) revealed that the 3isolates belonged to the QX-type (GI-19 lineage). Wholegenome sequencing and recombination analysis indi-cated that CZ200515 and CZ210840 contained geneticmaterial from 4/91 and Scyz3 (QX-type), possibly dueto recombination between the circulating strain and the4/91 vaccine strain, while no evidence of recombinationwas found in CZ211063. Pathogenicity analysis in 1-day-old specific pathogen-free (SPF) chickens demon-strated that all 3 isolates caused severe tissue damageand varying degrees of mortality. Virus cross-neutraliza-tion assay revealed decreased antigen relatednessbetween the isolates and the QX-type vaccine strain(QXL87). Amino acid sequence homology analysis ofS1 revealed 5%-6.5% variances between the isolates andQXL87. Analysis of the S1 subunit structure revealedthat mutations of amino acid residues in the hypervari-able region (HVR) and the neutralizing epitope regionresulted in antigenic variation in isolates by changingthe antigen conformation. Our data indicate antigenic-ity variances between QX isolates and QXL87 vaccinestrains, potentially resulting in immune evasion occur-rences. Overall, these results offer crucial insights intothe epidemiology and pathogenicity of QX-type IBV,facilitating improved selection and formulation of vac-cines for disease management.