文献类型： 外文期刊

作者： Zhao, Bingru ¹ ; Suo, Langda ³ ; Wu, Yujiang ³ ; Chen, Tong ² ; Tulafu, Hanikezi ² ; Lu, Qingwei ² ; Liu, Wenna ² ; Sammad, Abdul ⁵ ; Wu, Cuiling ⁶ ; Fu, Xuefeng ² ;

作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, Jiangsu Livestock Embryo Engn Lab, Nanjing 210095, Jiangsu, Peoples R China

2.Xinjiang Acad Anim Sci, Inst Anim Sci, Key Lab Genet Breeding & Reprod Xinjiang Wool Shee, Urumqi 830011, Xinjiang, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Inst Anim Sci, Lhasa 850009, Xizang, Peoples R China

4.Xinjiang Agr Univ, Coll Anim Sci, Urumqi 830052, Xinjiang, Peoples R China

5.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

6.Xinjiang Normal Univ, Int Ctr Collaborat Management Cross Border Pest Ce, Key Lab Special Environm Biodivers Applicat & Regu, Sch Life Sci, Urumqi 830017, Xinjiang, Peoples R China

关键词： Tibetan cashmere goat; Stress adaptation; Metabolite; Multi-omics analysis; KEGG pathways

期刊名称：GENOMICS （ 影响因子：4.4； 五年影响因子：4.2 ）

ISSN： 0888-7543

年卷期： 2024 年 116 卷 2 期

页码：

收录情况： SCI

摘要： Tibetan cashmere goats are not only served as a valuable model for studying adaptation to hypoxia and highaltitude conditions but also playing a pivotal role in bolstering local economies through the provision of premium quality cashmere yarn. In this study, we performed an integration and network analysis of metabolomic, transcriptomic and proteomic to elucidate the role of differentially expressed genes, important metabolites, and relevant cellular and metabolic pathways between the fine (average 12.04 +/- 0.03 mu m of mean fiber diameter) and coarse cashmere (average 14.88 +/- 0.05 mu m of mean fber diameter) producing by Tibetan cashmere goats. We identified a distinction of 56 and 71 differential metabolites (DMs) between the F and C cashmere groups under positive and negative ion modes, respectively. The KEGG pathway enrichment analysis of these DMs highlighted numerous pathways predominantly involved in amino acid and protein metabolism, as indicated by the finding that the most impactful pathway was the mammalian target of rapamycin (mTOR) signalling pathway. In the F group, we identified a distinctive metabolic profile where amino acid metabolites including serine, histidine, asparagine, glutamic acid, arginine, valine, aspartic acid, tyrosine, and methionine were upregulated, while lysine, isoleucine, glutamine, tryptophan, and threonine were downregulated. The regulatory network and gene co-expression network revealed crucial genes, metabolites, and metabolic pathways. The integrative omics analysis revealed a high enrichment of several pathways, notably encompassing protein digestion and absorption, sphingolipid signalling, and the synaptic vesicle cycle. Within the sphere of our integrative analysis, DNMT3B was identified as a paramount gene, intricately associated with significant proteins such as HMCN1, CPB2, GNG12, and LRP1. Our present study delineated the molecular underpinnings governing the variations in cashmere characteristics by conducting comprehensive analyses across metabolomic, transcriptomic, and proteomic dimensions. This research provided newly insights into the mechanisms regulating cashmere traits and facilitated the advancement of selective breeding programs aimed at cultivating high-quality superfine Tibetan cashmere goats.