Recombinant pseudorabies virus expressing P12A and 3C of FMDV can partially protect piglets against FMDV challenge
文献类型： 外文期刊
作者： Zhang, Keshan 1 ; Huang, Jiong 3 ; Wang, Qingang 1 ; He, Yannan 1 ; Xu, Zhuofei 1 ; Xiang, Min 1 ; Wu, Bin 1 ; Chen, Huan 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Agr Sci, Minist Agr, Lanzhou Vet Res Inst,Key Lab Anim Virol, State Key Lab Vet Etiol Biol,Natl Foot & Mouth Di, Lanzhou 730046, Gansu, Peoples R China
3.Xinjiang Acad Anim Sci, Inst Vet Med, Urumqi 830000, Peoples R China
关键词： 3C protease: expression;EC 3.4.22.28;capsid precursor polypeptide: P12A;expression;PRV-P12A3C: immunologic-drug;immunostimulant-drug;efficacy;disease severity;humoral immune response;cellular immune response;P12A3C immunogenicity
期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）
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收录情况： SCI
摘要： One of the crucial factors for evaluation of an effective genetically engineered vaccine is whether susceptible animals are protected from virus challenge after vaccination. In this study, a recombinant pseudorabies virus (PRV-P12A3C) that expressed capsid precursor polypeptide P12A and nonstructural protein 3C of foot-and-mouth disease virus (FMDV) was used as a vaccine. The expression of P12A3C and its immunogenicity and protective efficacy against FMDV challenge were measured. Humoral and cellular immune responses were evaluated after each immunization. Subsequently, each piglet was challenged with 1000 ID50 (50% infection dose) FMDV serotype O, named OR180, which is used to produce vaccine in China. PRV-P12A3C induced a high level of neutralizing antibody and FMDV-specific lymphocytes. Inactivated vaccine provided 100% protection, and the vector strain (TK-/gE(-)/gl(-)) showed no protection. PRV-P12A3C induced 60% protection, compared with piglets that were vaccinated with TK-/gE(-)/gl(-). The severity of clinical signs for the remaining two piglets was lighter and the appearance of vesicles was delayed
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