文献类型： 外文期刊
作者： Hu, Shengwei 1 ; Qiao, Jun 1 ; Fu, Qiang 1 ; Chen, Chuangfu 1 ; Ni, Wei 2 ; Wujiafu, Sai 1 ; Ma, Shiwei 1 ; Zhang, Hui 1 ; S 1 ;
作者机构： 1.Shihezi Univ, Coll Life Sci, Shihezi, Peoples R China
2.Shihezi Univ, Coll Anim Sci, Shihezi, Peoples R China
3.Xinjiang Acad Anim Sci, Inst Vet Med, Urumqi, Peoples R China
4.Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130023, Peoples R China
期刊名称：ELIFE （ 影响因子：8.14； 五年影响因子：9.056 ）
ISSN： 2050-084X
年卷期： 2015 年 4 卷
页码：
收录情况： SCI
摘要： Foot-and-mouth disease virus (FMDV) is an economically devastating viral disease leading to a substantial loss to the swine industry worldwide. A novel alternative strategy is to develop pigs that are genetically resistant to infection. Here, we produce transgenic (TG) pigs that constitutively expressed FMDV-specific short interfering RNA (siRNA) derived from small hairpin RNA (shRNA). In vitro challenge of TG fibroblasts showed the shRNA suppressed viral growth. TG and non-TG pigs were challenged by intramuscular injection with 100 LD50 of FMDV. High fever, severe clinical signs of foot-and-mouth disease and typical histopathological changes were observed in all of the non-TG pigs but in none of the high-siRNA pigs. Our results show that TG shRNA can provide a viable tool for producing animals with enhanced resistance to FMDV.
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