您好，欢迎访问新疆畜牧科学院 机构知识库！

A gene family from Echinococcus granulosus differentially expressed in mature adult worms

文献类型： 外文期刊

作者： Zhang, WB 1 ; Li, J 2 ; You, H 2 ; Zhang, ZZ 2 ; Turson, G 2 ; Loukas, A 2 ; McManus, DP 2 ;

作者机构： 1.Univ Queensland, Queensland Inst Med Res, Australian Ctr Int & Trop Hlth & Nutr, Mol Parasitol Lab, Brisbane, Qld 4029, Australia

2.Univ Queensland, Queensland Inst Med Res, Australian Ctr Int & Trop Hlth & Nutr, Mol Parasitol Lab, Brisbane, Qld 4029, Australia; Xinjiang Acad Anim Sci, Vet Res Inst, Urumqi 830000, Xinjiang, Peoples R China

关键词： Echinococcus granulosus;differential gene expression;differential display;DDRT-PCR;RT-PCR;gene family

期刊名称：MOLECULAR AND BIOCHEMICAL PARASITOLOGY （ 影响因子：1.759； 五年影响因子：2.135 ）

ISSN： 0166-6851

年卷期： 2003 年 126 卷 1 期

页码：

收录情况： SCI

摘要： Differences in mRNA expression between immature adult worms (IAW) and mature adult worms (MAW) of Echinococcus granulosus were determined using polymerase chain reaction-based differential display (DDRT-PCR). Twenty-eight putative differential cDNA fragments were isolated, cloned and sequenced. mRNAs from IAW and MAW were probed with the labelled fragments. Six cDNA fragments (coded as egM12, egM13, egM22, egM26, egM30 and egM34) were putatively determined to be specific to MAW by Northern hybridisation. The stage-specificity of egM12, egM13 and egM34 was confirmed by RT-PCR. RNAs of IAW, MAW, protoscoleces and oncospheres, probed with egM13 and egM30, showed that the mRNAs were expressed exclusively in MAW, which implied involvement in the regulation of egg development. Using the labelled fragments to screen a cDNA library of MAW, 99 clones were identified and analysed. An alignment of selected clones showed that the MAW-specific mRNAs belonged to a family. Examination of the deduced amino acid sequence of three of the corresponding cDNAs (egM4, egM9 and egM123) indicated they were cysteine-rich and contained a 24 amino acid repeat sequence, repeated four to six times. The repeat regions were predominantly alpha helical in nature with interspersed turns, forming alternating zones of positive and negative charge. The functional significance of each of the cDNAs identified is unclear as none had significant sequence similarity to genes of known function. However, polypeptides encoded by egM4 and egM123 were recognised by antibodies in a serum pool from dogs experimentally infected with E. granulosus, suggesting they could prove of value in serodiagnosis of definitive hosts. (C) 2002 Elsevier Science B.V. All rights reserved.

  • 相关文献

[1]Vaccination of bovines against Echinococcus granulosus (cystic echinococcosis). Heath, David D.,Robinson, Christine,Shakes, Trevor,Huang, Yan,Gulnur, Tursun,Shi, Baoxing,Zhang, Zhuangzhi,Anderson, Garry A.,Lightowlers, Marshall W..

[2]Further studies on an intermediate host murine model showing that a primary Echinococcus granulosus infection is protective against subsequent oncospheral challenge. Zhang, WB,You, H,Zhang, ZZ,Turson, G,Hasyet, A,McManus, DP.

[3]Epidemiology and control of echinococcosis in central Asia, with particular reference to the People's Republic of China. Zhang, Wenbao,Li, Jun,Wen, Hao,Zhang, Wenbao,Li, Jun,McManus, Donald P.,Zhang, Zhuangzhi,Shi, Baoxin,Wu, Weiping,Zhou, Xiaonong. 2015

[4]Solid lipid nanoparticle suspension enhanced the therapeutic efficacy of praziquantel against tapeworm. Xie, Shuyu,Pan, Baoliang,Wang, Ming,Zhou, Wenzhong,Shi, Baoxin,Zhang, Zhuangzhi,Zhang, Xu. 2011

[5]Genome-wide sequencing of small RNAs reveals a tissue-specific loss of conserved microRNA families in Echinococcus granulosus. Bai, Yun,Jin, Lei,Kang, Hui,Zhu, Yongqiang,Zhang, Lu,Li, Xia,Ma, Fengshou,Wang, Shengyue,Zhang, Zhuangzhi,Zhao, Li,Shi, Baoxin,Li, Jun,Zhang, Wenbao,McManus, Donald P.. 2014

[6]Functional expression and characterization of Eehinococcus granulosus thioredoxin peroxidase suggests a role in protection against oxidative damage. Li, J,Zhang, WB,Loukas, A,Lin, RY,Ito, A,Zhang, LH,Jones, M,McManus, DP. 2004

[7]Cloning and characterization of an Echinococcus granulosus ecdysteroid hormone nuclear receptor HR3-like gene. Yang, Mei,Zhang, Fuchun,Li, Jun,Wang, Hui,Guo, Baoping,Wu, Chuanchuan,Shou, Xi,Yang, Ning,Zhang, Wenbao,Yang, Mei,Wu, Jun,Zhang, Zhuangzhi,McManus, Donald P.. 2017

[8]Immunoglobulin profiles in a murine intermediate host model of resistance for Echinococcus granulosus infection. Zhang, WB,You, H,Li, J,Zhang, ZZ,Turson, G,Aili, H,Wang, JC,McManus, DP. 2003

[9]用半定量RT-PCR法检测BⅢB4基因在绵羊皮肤组织中的mRNA表达. 劳荃蘅,黄锡霞,田可川,徐新明,贺三刚,张廷虎,吴伟伟,刘祯. 2009

[10]苏博美利奴羊S100A11基因序列分析及表达研究. 付雪峰,刘春洁,黄锡霞,石刚,徐新明. 2016

[11]蓝舌病新疆分离株VP7蛋白编码基因序列分析及一步法RT-PCR方法的建立. 马晓菁,谷文喜,叶锋,刘帅,刘丽娅,谢彩云,钟旗,易新萍. 2024

[12]新吉细毛羊角蛋白的EST筛选及表达研究. 刘春洁,付雪峰,田可川,黄锡霞,徐新明,詹振宏,于丽娟. 2015

[13]新疆某地区犬流感病毒血清学及病原学调查. 米晓云,盛肖刚,吴建勇,汪萍,杨学云,魏玉荣. 2021

[14]Hoxc13基因在苏博美利奴羊胎羔生长发育期不同组织中的表达分析. 杜建文,何军敏,陈春艳,田月珍,徐新明,付雪峰,哈尼克孜·吐拉甫,赵冰茹,朱桦,黄锡霞,田可川. 2019

[15]鉴别蓝舌病病毒血清29型RT-PCR与qRT-PCR方法的建立与应用. 李占鸿,宋子昂,谷文喜,李华春,钟旗,杨振兴,李卓然,廖德芳,杨恒. 2022

[16]狂犬病病毒SRV_9株N、P、G和L蛋白基因的克隆及表达载体构建. 魏玉荣,易忠,符子华,马素贞,王晓萍,简子健,魏婕,王海烽,朱晶晶. 2009

[17]中国美利奴羊(新疆型)皮肤组织GAPDH基因实时荧光定量PCR方法的建立. 田月珍,黄锡霞,狄江,田可川,吴伟伟,徐新明,哈尼克孜,付雪峰,张艳花,马依拉,艾买提. 2012

[18]禽流感病毒新疆株A/Duck/XJ/4(H5N1)的PB1、PB2和PA基因测序及进化分析. 汪萍,沙依兰古丽,符子华,盛卓君,巴哥得力,陆桂丽,成进. 2008

[19]绵羊卵丘细胞直径与卵母细胞质量的相关性. 古丽米热·阿布都热依木,吴阳升,林嘉鹏,汪立芹,张利,蒋香菊,杨楠,唐淑红,黄俊成. 2014

[20]2016年新疆尉犁县羊蓝舌病的诊断. 丁梦玥,马晓菁,叶锋,刘帅,薛新梅,宋迎春,加帕尔·哈斯木,易新萍,刘丽娅,孟肖潇,马俊杰,谷文喜,钟旗. 2018

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆畜牧科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台