文献类型： 外文期刊

作者： Yu, Xinyu ¹ ; Xieripu, Adilijiang ¹ ; Xu, Qilan ¹ ; Zulipikaer, Azhati ² ; Song, Yiyan ¹ ; Cai, Ling ¹ ; Chen, Jin ¹ ;

作者机构： 1.Nanjing Med Univ, Sch Publ Hlth, Longmian Ave 101, Nanjing 211166, Jiangsu, Peoples R China

2.Xinjiang Acad Anim Sci, Urumqi 830011, Xinjiang, Peoples R China

3.Nanjing Med Univ, Key Lab Modern Toxicol, Minist Educ, Sch Publ Hlth, Nanjing 211166, Jiangsu, Peoples R China

4.Nanjing Med Univ, Ctr Global Hlth, Sch Publ Hlth, Nanjing 211166, Jiangsu, Peoples R China

5.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

关键词： camel serum albumin; nanoparticles; self-assembly; redox-responsive; synergistic effect

期刊名称：JOURNAL OF BIOMEDICAL RESEARCH （ 影响因子：2.3； 五年影响因子：2.3 ）

ISSN： 1674-8301

年卷期： 2020 年 34 卷 1 期

页码：

收录情况： SCI

摘要： The aim of this study was to prepare camel serum albumin (CSA) nanoparticles using a self-assembly strategy to co-immobilize curcumin (CCM) and doxorubicin (Dox) which was in favor of combined chemotherapy and biomedical applications of bactrian (Camelus bactrianus) CSA. The constructed CSA nanoparticles (CSA-NPs) with the size around 200 nm displayed a high degree of polydispersity and further encapsulation of CCM and Dox caused no apparent morphological changes to the nanocomposite (CCM/Dox CSA-NPs). The synergistic cytotoxic effect of CCM and Dox on cancer cell A549 was observed with the calculated combination index less than 1.0. Moreover, the release kinetic profile of encapsulated drugs showed a concentration dependence of glutathione (GSH) originating from the GSH used in nanoparticle formation to break the intramolecular disulfide bonds. In vitro cytotoxicity evaluations also revealed that CCM/Dox CSA-NPs showed higher cytotoxicity than that of single drug loaded CSA-NPs, which was also validated by high content screen assay. Taken together, the CCM/Dox CSA-NPs with redox-responsive attributes provided an integrated protein-based combinational drug-delivery matrix to exert synergistic effects.