Pre-evaluation of humoral immune response of Bactrian camels by the quantification of Th2 cytokines using real-time PCR
文献类型： 外文期刊
作者： Yu, Xinyu 1 ; Wu, Yuan 2 ; Zhang, Jiarong 1 ; Jirimutu 3 ; Zulipikaer, Azhati 4 ; Chen, Jin 1 ;
作者机构： 1.Nanjing Med Univ, Ctr Global Hlth, Sch Publ Hlth, Nanjing 211166, Jiangsu, Peoples R China
2.Nanjing Med Univ, Jiangsu Canc Hosp, Jiangsu Inst Canc Res, Dept Med Oncol,Affiliated Canc Hosp, Nanjing 210009, Jiangsu, Peoples R China
3.Inner Mongolia Agr Univ, Coll Food Sci & Engn, Key Lab Dairy Biotechnol & Bioengn, Minist Educ, Hohhot 010018, Inner Mongolia, Peoples R China
4.Xinjiang Acad Anim Sci, Urumqi 830011, Xinjiang, Peoples R China
5.Nanjing Med Univ, Key Lab Modern Toxicol, Sch Publ Hlth, Minist Educ, Nanjing 211166, Jiangsu, Peoples R China
6.Nanjing Med Univ, Key Lab Antibody Tech Natl Hlth Commiss, Nanjing 211166, Jiangsu, Peoples R China
关键词： Bactrian camels; Th2 cytokines; humoral immune response; real-time PCR
期刊名称：JOURNAL OF BIOMEDICAL RESEARCH （ 影响因子：2.3； 五年影响因子：2.3 ）
ISSN： 1674-8301
年卷期： 2020 年 34 卷 5 期
页码：
收录情况： SCI
摘要： With the increasing immunological studies on camels due to the advantage of their single-chain antibodies for humanizations, it is demanding to develop an easy-to-handle evaluation method of their humoral immune response before proceeding with immunization of foreign antigens that may be toxic to camels. In this study, we quantitatively determined the expression levels of T-helper 2 (Th2) cytokines in peripheral blood lymphocytes obtained from Bactrian camels by real-time PCR. The recorded kinetic profiles resulting from the immunization of ovalbumin (OVA) indicated that after immunization, Th2 cytokines including interleukin (IL) families such as IL-4, IL-10, and IL-13 in the camels were up-regulated by a factor of 1.78, 3.15, and 1.22, respectively, which was validated by traditional enzyme-linked immunosorbent assay (ELISA) methods. Unlike ELISA which requires specific enzyme-labeled antibodies, this established method based on the minimal amount of blood samples holds an advantage in the preliminary evaluation of camel humoral immune response with desirable precision, which is meaningful for biomedical explorations of camel-derived antibodies.
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