Novel strategy for Poxviridae prevention: Thermostable combined subunit vaccine patch with intense immune response
文献类型： 外文期刊
作者： Wen, Yuan 1 ; Deng, Shuyue 3 ; Wang, Tianmin 1 ; Gao, Mengtian 1 ; Nan, Wenlong 4 ; Tang, Fang 1 ; Xue, Qinghong 5 ; Ju, Yanmin 3 ; Dai, Jianjun 1 ; Wei, Yurong 6 ; Xue, Feng 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China
2.Nanjing Agr Univ, Sanya Inst, Sanya 572025, Peoples R China
3.China Pharmaceut Univ, Coll Pharm, Nanjing 211198, Peoples R China
4.China Anim Hlth & Epidemiol Ctr, Lab Diag Dev, 369 Nanjing Rd, Qingdao 266032, Peoples R China
5.China Inst Vet Drug Control, Beijing 100081, Peoples R China
6.Xinjiang Acad Anim Sci, Inst Vet Med, Xinjiang Key Lab Anim Infect Dis, Urumqi 830099, Peoples R China
关键词： Combined subunit vaccine (CSV); Lumpy skin disease virus (LSDV); Poxviruses; Microneedle; Vaccine stability; Neutralizing antibody
期刊名称：ANTIVIRAL RESEARCH （ 影响因子：4.5； 五年影响因子：5.0 ）
ISSN： 0166-3542
年卷期： 2024 年 228 卷
页码：
收录情况： SCI
摘要： Poxviruses gained international attention due to the sharp rise in monkeypox cases in recent years, highlighting the urgent need for the development of a secure and reliable vaccine. This study involved the development of an innovative combined subunit vaccine (CSV) targeting poxviruses, with lumpy skin disease virus (LSDV) serving as the model virus. To this end, the potential sites for poxvirus vaccines were fully evaluated to develop and purify four recombinant proteins. These proteins were then successfully delivered to the dermis in a mouse model by utilizing dissolvable microneedle patches (DMPs). This approach simplified the vaccination procedure and significantly mitigated the associated risk. CSV-loaded DMPs contained four recombinant proteins and a novel adjuvant, CpG, which allowed DMPs to elicit the same intensity of humoral and cellular immunity as subcutaneous injection. Following immunization with SC and DMP, the mice exhibited notable levels of neutralizing antibodies, albeit at a low concentration. It is noteworthy that the CSV loaded into DMPs remained stable for at least 4 months at room temperature, effectively addressing the storage and transportation challenges. Based on the study findings, CSV-loaded DMPs are expected to be utilized worldwide as an innovative technique for poxvirus inoculation, especially in underdeveloped regions. This novel strategy is crucial for the development of future poxvirus vaccines.
- 相关文献
作者其他论文 更多>>
-
Emergence of a Novel G4P[6] Porcine Rotavirus with Unique Sequence Duplication in NSP5 Gene in China
作者：Zhou, Xia;Hou, Xueyan;Xiao, Guifa;Liu, Bo;Jia, Handuo;Zhai, Shao-Lun;Hou, Xueyan;Guo, Qingyong;Wei, Jie;Mi, Xiaoyun;Wei, Yurong
关键词：rotavirus; porcine; zoonosis; duplicated sequence; genomic rearrangement; NSP5
-
Antibiofilm activity of polyethylene glycol-quercetin nanoparticlesloaded gelatin-N,O-carboxymethyl chitosan composite nanogels against Staphylococcus epidermidis
作者：Luo, Wanhe;Jiang, Yongtao;Liu, Jinhuan;Liu, Jinhuan;Sun, Beibei;Gao, Xiuge;Guo, Dawei;Algharib, Samah Attia;Wei, Jie;Wei, Yurong
关键词：Staphylococcus epidermidis; quercetin; biofilm; nanogels; on-demand release
-
Enhanced Cellular Delivery of Tildipirosin by Xanthan Gum-Gelatin Composite Nanogels
作者：Li, Xianqiang;Dou, Jinfeng;Xiong, Youyi;Liu, Jinhuan;Jiang, Yongtao;Luo, Wanhe;Liu, Jinhuan;Luo, Wanhe;Li, Chao;Algharib, Samah;Li, Chao;Algharib, Samah;Algharib, Samah;Dawood, Ali;Wei, Jie;Wei, Yurong
关键词：
-
Recombinant Human Adenovirus Type 5 Co-expressing RABV G and SFTSV Gn Induces Protective Immunity Against Rabies Virus and Severe Fever With Thrombocytopenia Syndrome Virus in Mice
作者：Zhao, Zhongxin;Zheng, Wenwen;Yan, Lina;Xu, Tong;Liu, Lele;Tian, Li;Zheng, Xuexing;Sun, Peilu;Zhu, Yelei;He, Hongbin;Wei, Yurong
关键词：severe fever with thrombocytopenia syndrome virus; rabies virus; adenovirus type 5; bivalent vaccine; virus-neutralizing antibody
-
Molecular Epidemiology and Characteristics of Streptococcus agalactiae Isolated from Bovine Mastitis in Large Dairy Herds of China
作者：Yang, Xueyun;Jia, Bin;Yang, Xueyun;Wang, Dengfeng;Lie, Jianjun;Menge, Xiaoxiao;Wei, Yurong;Tuerxun, Gunuer;Jin, Yinghong;Xue, Jing;Ali, Tariq;Ali, Tariq;Han, Bo
关键词：Antibiotic resistance; Bovine mastitis; Capsular serotyping; Genotype; Streptococcus agalactiae; Virulence genes
-
Genetic Characterization of a Novel Mutant of Peste Des Petits Ruminants Virus Isolated from Capra ibex in China during 2015
作者：Zhu, Zixiang;Du, Xiaoli;Zhang, Xiangle;Li, Pengfei;Liu, Xiangtao;Zheng, Haixue;Zhang, Xiaocui;Huang, Jiong;Adili, Gulizhati;Zheng, Xueguang;Xue, Qinghong
关键词：
-
Genetically modified rabies virus vector-based bovine ephemeral fever virus vaccine induces protective immune responses against BEFV and RABV in mice
作者：Zheng, Wenwen;Zhao, Zhongxin;Tian, Li;Liu, Lele;Xu, Tong;Zheng, Ye;Zheng, Xuexing;Wang, Xianwei;He, Hongbin;Xia, Xianzhu;Wei, Yurong
关键词：bivalent vaccine; bovine ephemeral fever virus; G protein; rabies virus
