文献类型： 外文期刊

作者： Abudoukerimu, Azierguli ¹ ; Hasimu, Axiangu ⁴ ; Abudoukerimu, Abudouhabaer ⁵ ; Tuerxuntuoheti, Gulijiannaiti ⁶ ; Huang, Yixin ² ; Wei, Jie ⁷ ; Yu, Tao ⁸ ; Ma, Hong ³ ; Yimiti, Delixiati ¹ ;

作者机构： 1.Xinjiang Med Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, Urumqi 830017, Peoples R China

2.Xinjiang Med Univ, Sch Basic Med Sci, Dept Microbiol, Urumqi 830017, Peoples R China

3.Xinjiang Key Lab Mol Biol Endem Dis, Urumqi 830017, Peoples R China

4.Xinjiang Med Univ, Sch Basic Med Sci, Dept Pathol, Urumqi 830017, Peoples R China

5.Hotan Normal Coll, Dept Sci, Hotan 848000, Xinjiang Uygur, Peoples R China

6.Hotan Normal Coll, Dept Linguist, Hotan 848000, Xinjiang Uygur, Peoples R China

7.Xinjiang Acad Anim Sci, Inst Vet Med, Urumqi 830000, Xinjiang Uygur, Peoples R China

8.Shandong First Med Univ, Shandong Inst Parasit Dis, Jinan 272033, Shandong Provin, Peoples R China

9.Shandong Acad Med, Shandong Inst Parasit Dis, Jinan 272033, Shandong, Peoples R China

期刊名称：JOURNAL OF ONCOLOGY （ 影响因子：4.501； 五年影响因子：4.463 ）

ISSN： 1687-8450

年卷期： 2022 年 2022 卷

页码：

收录情况： SCI

摘要： Cervical carcinoma is one of the serious pernicious cancers that influence women's health. Invasion and metastasis are the chief reason of poor prognosis of cervical carcinoma. Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is a significant regulatory factor of intracellular oxygen supersession, and its expression or increased activity is closely related to the arise and expansion of various human tumors. However, the relationship between HIF-1 alpha (hypoxia-inducible factor 1) and Hippo pathway target gene Yes-related protein (YAP) and transcriptional coactivator (TAZ) in cervical carcinoma remains unclear. Here, we studied the clinical correlation of HIF-1 alpha and YAP/TAZ expression in normal tissues, cervical intraepithelial neoplasia (CIN), and cervical squamous cell carcinoma (CSCC). In order to analyze the role of HIF-1 alpha in CCSC in vitro, SiHa cells with high expression of HIF-1 alpha and C33a cells with low expression of HIF-1 alpha were screened by detection. After transfection with lentivirus, HIF-1 alpha levels were downregulated in SiHa cells and upregulated in C33a Cells, respectively. Then, the expression of HIF-1 alpha in transfected cervical cancer cells Siha and C33a was detected by qRT-PCR and Western blot, and the expression of YAP/TAZ was detected in cervical squamous cell carcinoma cells after HIF-1 alpha expression was altered. To explore HIF-1 alpha role in cell proliferation, invasion, and metastasis, we examined the changes of cell function in cervical cancer cells with HIF-1 alpha overexpression and inhibition by MTT assay, wound healing assay, Transwell test, and other cell function tests. At the same time, HIF-1 alpha overexpression and HIF-1 alpha inhibition cervical cancer cells were transplanted into nude mice, and tumors were isolated from the nude mice, and tumor volume and weight were observed. In conclusion, HIF-1 alpha significantly promotes the proliferation, invasion, and migration of cervical carcinoma cells by upregulating YAP/TAZ. In addition, YAP/TAZ, the target gene of Hippo pathway, plays an important role in CCSC cells, pointing out that HIF-1 alpha is provided with treatment potential for the treatment of CCSC.